SLE Cartridge-Supported Liquid Extraction

The SLE Cartridge (Supported Liquid Extraction) manufactured from Hawach Scientific is a sample preparation methodology with features of high efficiency, economy. There only need 2 step protocol (Sample Loading| Elution) when the analytes needed to be extracted from aqueous based samples.

The multi-porous diatomaceous earth which is with high surface area and lower chemical reaction were packed as stationary phase for the SLE in SLE extraction. The aqueous based sample passes through diatomaceous earth beads when sample loading, it can forming a thin membrane via adsorb and capillary action. When Elution, the organic phase which incompatible with water will flow through surface of aqueous phase then forming and aqueous-organic interface. The analytes partition with very high efficiency between these two phases.

Hawach SLE Cartridge Features:
Shorten extraction time and simplified process
Excellent reproducibility and higher recovery rate
Parallel manipulation and automation will be easy
Reducing cost by lessen the amount of organic solvent
Unnecessary for vigorous shaking and no emulsion formation exist

Hawach SLE Cartridge Application:
SLE is widely used in environmental protection, food safety, forensic science clinical diagnosis, and material inspection area
It can be used for the parabens determination in pharmaceuticals, cosmetics and foodstuffs
It can be used in the analysis of drug metabolites in biological fluids

PES Membrane Filter Introduction

When you mentioned about the membrane filter, you know that there are many materials such as Nylon, PTFE, PES, MCE, CA, PVDF, today, we want to introduce you our PES Membrane filters:

Our PES Membrane filter is Hydrophilic with extremely low extractables and low backgrounding, it can be used for the filtration of very board solvent compatibility, suitable for filtration of compatible organic solvents and aqueous samples. Low in extractables, very high filtration speed.

As it is resistant to a wide range of solvents, recommended for life science and pharmaceutical applications.

Features:
Lowest protein binding and extractables
Fast Flow Rate
High-throughput characteristics
Better chemical resistance than Cellulose acetate
Applications:
Life science
Pharmaceutical applications

Here is the normal pore size and diameter:
Pore size: 0.1um, 0.22um, 0.45um,0.8um, 1.0um, 3.0um, 5.0um, 10.0um

Diameter: 13mm, 25mm, 47mm, 50mm, 60mm, 90mm, 142mm, 150mm, 200mm, 293mm, 300mm

We have a round disc type and Roll type, accept OEM Products and customized size, very flexible sales policy, any question or help you need, please don’t hesitate to contact us.

Top 10 Misunderstandings of HPLC and UHPLC Columns (part3)

The guard column is not necessary

There are many benefits to using a guard column. First, the guard column prevents chemicals or particles from damaging the analytical column. Second, replacing a 5mm guard column requires only a small cost compared to replacing an expensive analytical column. Modern guard columns have the advantage of virtually no dead volume, fast replacement, and high pressure for UHPLC.

You can’t reverse the HPLC column to flush out the particles.

In fact, the HPLC column fill pressure is much higher than the maximum use pressure (usually 2 times higher). If a proper homogenizer is used for the column and a time is allocated to stabilize the bed, a well-filled column can be used in both directions.

An exception to the reverse use of the column is that the manufacturer uses a larger aperture plate at the injection end of the column, and reverse use may flush the packing out of the bed. If the manufacturer uses a high porosity frit at the inlet of the column, then backflushing the column may flush the column packing from the packed bed. When the column is packed at the factory, the sieve opening at the outlet end must be smaller than the smallest particle size in the column. For example, the average particle size of the chromatographic packing is 5 μm, the particle size distribution range is 3-7 μm, and the pore size of the outlet end sieve plate must be less than 3μm, so that the filler is not likely to run from the bed to the outside of the column. Most manufacturers choose a sieve plate with a pore size of 2 μm. As for the column screens at both ends of some manufacturers, the aperture is not the same. Generally, the injection end is larger and the sample end is smaller. Therefore, some manufacturers place an arrow indication at the column label indicating that it must be used in only one direction. There is certainly a possibility, so a chromatographer should read the column manual or instructions, or determine with the manufacturer whether the column can be backflushed.

The smaller the particle size of the column packing, the higher the pressure, the better the separation effect.

Ultra-small particle size and ultra-high column pressure are not necessarily the best choice for chromatographers!

Column characteristics studies of modern columns have led to new methods to evaluate the performance of a column. For example, a column with a new superficially porous material has the same column efficiency as the sub-2μm UHPLC column, but the column pressure is very low compared to conventional LC packed columns.

Column pressure does not affect chromatographic separation

Many parameters of the chromatogram are affected by column pressure, including the molar volume of some solute, stagnation volume, column porosity, retention factor, mobile phase density, dielectric constant, stationary phase structure, pH and ionization constant. Why does column pressure cause more and more attention? The reason is that many commercially available chromatographs are ultra-high pressure chromatographs and columns. When the column is operated at a pressure of about 2000 psi (13.789 MPa), even if there is a small difference in retention time, it does not attract our attention; especially if the repeatability is good and the quantification is not affected. However, when the column pressure is close to 2000 psi (13.789 MPa), the effect of column pressure may be quite significant.

Carbon Block Filter Cartridge(CTO)

Hawach carton block filter cartridge is used by special technology. With high-quality powdered activated carbon, which is produced through special low-temperature compression activation manufacturing process.

And the deep filtration effect makes the filter cartridge form ultramicro-pore, and the micron rating is tortuous, which is greatly prolongs the contact area between water and activated carbon, and effectively removes carcinogenic substances such as heterochromatic, odor, heavy metals and pesticide residues, and effectively suppresses them and bacterial propagation.

Activated carbon with high adsorption capacity and pore-forming property of powder binder can be fully utilized. Activated carbon can be formed by natural activation at high temperature to avoid the surface of activated carbon being encapsulated by binder to reduce its adsorption function and maximize the specific surface area and adsorption speed. The carbon block cartridge can withstand up to 10 kg of water pressure to ensure the purity and safety of water quality and make the water taste more delicious.

About the application:
Pre-filtration or pre-treatment in RO applications
Chlorine and odor removal
Color reduction

Headspace Vials and Seals for GC from Starlab Scientific Co., Ltd

First, how to choose a column
In modern high performance liquid chromatography, the separation effect depends largely on the choice of chromatographic packing. However, the choice of chromatographic packing is very wide. To make a suitable choice, you must have a certain understanding and understanding.
1. Normal phase chromatography
The stationary phase for normal phase chromatography is typically silica gel (Silica), as well as other bonded phase fillers having polar functional groups such as amine groups (NH2, APS) and cyano groups (CN, CPS).
Since the silicon hydroxy group (SiOH) or other groups on the surface of the silica gel are highly polar, the order of separation is based on the polarity of each component in the sample, that is, the component with the strong polarity is first washed out of the chromatogram. column. The phase of the mobile phase used in normal phase chromatography is relatively lower than that of the stationary phase, such as Hexane, Chloroform, Methylene Chloride, and the like.
2, reversed phase chromatography
Reversed phase chromatography packings are often based on silica gel with a bonded phase bonded to a relatively weakly polar functional group. The mobile phase used in reversed-phase chromatography is more polar, usually water, a mixture of buffer and methanol, and nitrile. The order in which the sample exits the column is that the more polar combination is first flushed out, while the less polar component will have a stronger retention on the column.

Commonly used reverse phase packings are C18 (ODS), C8 (MOS), C4 (B), C6H5 (Phenyl) and the like.

Second, the polymer filler

Most of the polymer seasonings are polystyrene-divinylbenzene or polymethylpropionate, and the main advantage is that it can be used at a pH of from 1 to 14. Compared with the silica matrix C18 filler, these fillers are more hydrophobic; macroporous polymer fillers are very effective for the separation of samples such as proteins. The disadvantage of current polymer fillers is that they are less efficient than silica matrix fillers.

Third, other inorganic fillers

Other HPLC inorganic filler columns have also been commercialized. Due to its special nature, it is generally limited to special uses. For example, graphitized carbon is also being used as a reversed phase chromatography packing. The separation of the filler is different from the alkylation phase of the silica gel matrix, and the surface of the graphitized carbon is the basis of retention, and no other surface modification is required. The pillar filler is generally more than an alkyl bonded silica gel or a porous polymer filler. The retention capacity is stronger, graphitized carbon can be used to separate certain geometrical conductors, and because the HPLC mobile phase does not dissolve, such columns can be used at any pH and temperature. Alumina can also be used in HPLC. Alumina particles are rigid and can be made into a stable column bed with the advantage of being used in mobile phases up to pH 12. However, due to the strong action of alumina and basic compounds, the application range is limited, so it is not widely used. The new zirconia filler can also be used in HPLC, commercialized polymer-coated porous zirconia microspheres. The column has a pH range of 1 to 14, and the application range is limited, so it is not widely used. The new zirconia filler can also be used in HPLC, a commercial polymer-coated porous zirconia microsphere column. Application PH range 1~14,
How to choose the filler particle size

At present, the commercial particle size is sold from 1um to over 30um. At present, the separation is mainly carried out with 3um, 5um and 10um fillers. The particle size of the filler mainly affects the two parameters of the packed column, namely column efficiency and back pressure. The smaller the particle size, the higher the column efficiency of the packed column; the less than 3um filler application, under the same selective conditions, the efficiency of the column can improve the resolution, but not the only factor. If the stationary phase is chosen correctly, but the resolution is not sufficient, it is useful to select a smaller particle size packing. The number of columns packed with 3um packing is nearly 30% higher than that of the 5um packing under the same conditions; however, 3um The back pressure of the color phase is twice that of 5um. At the same time, improved column efficiency means shorter columns can be selected under the same conditions to reduce analysis time. In addition, low viscosity solvents can be used as the mobile phase or the column temperature can be increased, such as acetonitrile instead of methanol. To reduce the pressure on the column.

Swimming Pool Filter Cartridge

The swimming pool filter cartridge is used to filter material with polyester fiber cloth. It has durable performance. Filter media, center rod and end cover are glued together as a whole to ensure better sealing of the end cover. Because it is a folding design, it provides a larger filter area, longer filter life and less filter core than other filter cores. The new times, which reduce the cost of filtration, is an economic filtering product from the viewpoint of industrial value.

Feature:
High flux medium has the advantages of high efficiency, low pressure loss and long life.
O- ring ensures the reliability of the filter.
It can withstand the working pressure difference of 0.24 MPa.
Folding surface design makes the pressure drop of high flow filter core lower and longer service life than others.
The filter shell can be equipped with a plurality of filter cartridge, which can be used for a wide range of flow, and can be used both in start-up and in continuous operation.
The filter cartridge is durable, and it can also configure filters at least and most economically.

What is the Difference for Our Three Series of Pipette?

We special produce 3 series of pipette and sales to the global market.
Standard series
Advanced series
Advanced plus series

The standard adjustable pipette only have half autoclavable (121 ℃) for choice, made from ABS757 housing and PVDF tip cone. The advanced series is for half autoclavable and Advanced plus series is for fully autoclavable. The fully autoclavable one made from PC housing and tips cones.

Price leveling is the same as function, the standard, the lowest. The advanced series the higher compared to standard. But all price level is favorable when compared with the world famous brand. (Eg, Eppendorf, GILSON, BRAND etc.)

Customized Flash Column by Global Clients’ Requirement

As a supplier who does chromatography consumables to global market, we do supply for standard Luer connection of the flash column. Both prepacked and empty column made from medical grade virgin PP material.

The column can be compatible with most of the flash system such as Biotage®, Armen®, Isco®. The empty cartridge can be available from column size of 4g to 330g, we also do customized color for customer requirement, such as blue and purple or others. The screw caps with o-rings and fruits work well and no leakage risk and easy to assemble. We can do stock under your requirement and after our mutual agreement.

Moreover, for customers do purification by their bonded phase, empty column is optical choice. For customers need prepacked cartridge, we do offer you with average Pore Size, particle size and high carbon content.

How Do You Know about High Liquid Performance Chromatography Column?

The high performance liquid chromatography column is a chromatography consumable that with the function for analysis and separation of mixture compounds. The working principle is to pump the analyte or say sample mixture in to a solvent(mobile phase such as water) under high pressure, as the column with stationary phase inside with chromatography packing sorbents, the column can do separation and identify the compounds which exist in any sample that could be dissolved into a kind of liquid in trace concentrations with the value as low as parts of per trillion.

As the versatility of the HPLC Column, the column can be used in a varieties of scientific and industrial applications such as pharmaceutical analysis, environmental analysis, forensic analysis, and chemicals analysis etc.

The sample retention( time ) always depending on the interaction between the stationary phase(Column inner packing material), the molecules being analyzed, and the solvent, or solvents used(liquid phase).

As the analytical sample passes through the column, it will have interactions between the two phases(stationary phase and mobile phase) at different rate, is primarily due to different polarities in the analytes. It means that the stationary phase with high polarity will interact with compounds with high polarity, so the molecules with lower polarity will have low retention and exit column earlier and appear peak faster.

Both Prepacked HPLC and empty HPLC Column, separately bulk sorbent available for your choice.（Empty Column picture as below for review）

What are Headspace Vials

Simply put, the headspace analysis is to analyze the gas present in the headspace vial. The laboratory vials used for headspace analysis are called headspace vials.
The essence of this experiment is that the volatile sample is heated and volatilized to form a gas in the space on the top of the sample vials and then diffused. Eventually allowing the top gas in the gas chromatograph vials above the volatile sample to enter the gas phase detection.
Adaptation: top gas chromatography
When the volatile or semi-volatile mixture has a higher boiling point, we need to heat it up to get it vaporized at the top. In this process, the liquid (solid or solid-liquid coexistence) sample is at the bottom, so the substance in the top gas can eventually be measured without touching the liquid in the sample bottle.
Top space gas chromatography is most suitable for the sample analysis of light component volatiles. This measurement technique can be used to analyze the gas in the top space of the liquid sample and apply to the solid dispersion method.
Top space gas analysis also helps in automated quality control or sample screening. This is a highly reproducible sample using modern instruments, enabling the entire experiment to be prepared for analysis in an efficient and accurate manner.
Application:
GC headspace technique is used to analyze the gasification of solid and liquid samples of volatile organic compounds. In recent years, the popularity of this technology has been recognized by laboratories around the world, especially for the analysis of residual organic solvents in alcohol, blood and pharmaceutical products.
Other common applications include industrial analysis, detection of volatile substances such as chemicals and plastics, flavor compounds in beverages and foods, perfumes and cosmetics.