What Is and How to Choose Non-Sterile Pipette Tips

 Non-sterile pipette tips are an indispensable tool in the hands of experimenters. Appropriate pipette tips can not only reduce the loss of pipettes but also improve the accuracy of experimental results. Here are some introductions to these non-sterile tips.

Non-sterile Pipette tips size:

• In general, in most lab consumable supplier companies, such as Hawach company, supply the volume of non-sterile tips is 10µL, 200µL, 300µL, 1000µL, etc.
• And different specifications of non-sterile tips have different lengths and calibers, but some of the same specifications of these pipette tips have exactly the same length.

Material:

• Tips with non-sterile are disposable consumables that come into direct contact with liquid samples and are made with PP material.
• And without a plasticizer release agent to the otherwise soluble matter will affect the experimental results

Feature of Non-sterile tip:

• No RNA, DNase, no heat source, ensure the integrity of the sample when moving, no pollution, no denaturation.
• Using USP-Class VI standard polystyrene raw materials, GMP100,000 clean workshop production
• And with a smooth inner wall, high-temperature resistance, less residual liquid
• In Hawach, supply bulk package packaging to meet customer needs
• And effectively avoid cross-contamination between samples caused by aerosol to match the pipettes of various brands and models on the market
• Especially tips with non-sterile can save the number of samples and reagents and improve the accuracy of experimental operations
• Adapt to the absorption of various organic solvents, and various pipettes
• The product complies with the country’s health standards and has passed the certification with no cytotoxicity, pyrogen-free.
• And the inner wall is smooth without flow marks, and the tip has no notch burrs, otherwise, it will lead to inaccurate drainage
• In addition,  material. At present, the tips with non-sterile on the market are basically made of polypropylene plastic (a colorless and transparent plastic with high chemical inertness and a wide temperature range).
• However, people usually make non-sterile pipette tips in different kinds of polypropylene: high-quality tips are often made from natural polypropylene, while cheaper tips may use recycled polypropylene plastic
• Pipette tips with non-sterile will not affect the accuracy of the pipetting results

Application:

• Designed non-sterile pipette tips for a variety of pipetting applications in molecular biology, cell culture, clinical laboratories, and more.
• The most commonly used type of tip is the non-sterile. And used in laboratory applications where sterility is not critical to the experiment or test being performed.
• In addition, widely used in molecular biology, gene sequencing, bioengineering, medicine, hospital clinical testing, and many other fields.
• Sterilized tips are usually boxed to prevent contamination. The concept of sterility refers to the absence of contamination from other microorganisms, such as fungi, bacteria, etc., the most difficult spores to discharge, unsterilized is the opposite
• And used for the experimental that avoids the presence or dissolution of fo reign biological contaminants to directly affect the analytical results

How to choose the  Non-sterile Pipette tip :

1. Weigh the sample volume

After rinsing the tips, repeat the sample loading procedure several times, weighing the sample each time and recording the reading. Calculate pipetting precision after converting test solution density to volume

2. Outer packaging inspection

The packaging should be intact and clearly marked.

3. Tip detection

• Check whether the suction head is deformed or damaged, and use a suitable sampler to suck up an appropriate amount of liquid to see if there is air leakage, suction hole blockage, or air leakage.
• After excluding the pipette factor, if there is any abnormality, that is, the batch of tips does not meet the experimental requirements of this laboratory.

 

There are also introduces about the function of disposable pipette tips, what is sterile filtered pipette tips, etc.

4 Ways to Choose Best Glass Fiber Filter Membranes

 As we all know, glass fiber filter membranes are laboratory filtration consumables, which have the characteristics of a high flow rate and strong dirt-holding capacity and are mainly for deep filtration. Today, here are some ways to choose them.

How to choose a glass fiber filter membrane:

1. According to size

• Domestic basic filter membranes are divided into fast, medium, and slow.
• Divided into finer ones, which generally have corresponding pore sizes, such as fiber filter membrane, the aperture: 0.45μm, 1.0μm, 1.6μm;
• Diameter: 13mm, 25mm, 47mm, 50mm, 60mm, etc;

2. Purity of material

• The quality of the filter membrane mainly depends on the purity of the cotton fiber, and it is best not to have additives.
• Glass fiber material filter paper is 99.90% glass fiber material.
• In addition, glass fiber filter paper has the characteristics of a high flow rate, strong dirt-holding capacity, and can filter fine particles.
• And compared with other fiber materials and man-made materials, glass fiber material filter paper has higher filtration efficiency and stronger dirt-holding capacity.
• Because most of the basic glass fiber filter paper does not use any additives, it is just curled glass microfibers, so it is very pure.
• But because of this, should pre-wash to avoid not to remain in the filtrate in the microfibers, and should add a surface filter under the glass fiber.

3. According to usage

• There are many types of surface filtration membranes, which are hydrophilic and hydrophobic (there are also some hydrophilic and hydrophobic dual-purpose membranes).
• In short, hydrophilicity is more suitable for filtering aqueous solutions, but not for filtering organic solvents, acid-base solutions, and air; hydrophobicity is suitable for filtering organic solvents, acid-base solutions, and air.
• But in fact, depending on the filter material, they have different characteristics.

4. Polypropylene vs. glass fiber membrane

• If it is general ambient air sampling, not sampling under high-temperature conditions, should not recommend trying a glass fiber membrane.
• High-throughput polypropylene fiber membrane, suitable for large-flow sampling
• Good hydrophobic performance, not easy to absorb water vapor in the air, good constant weight performance after repeated heating, can save repeated heating process
• Small resistance, high capture efficiency
• High strength, basically no shedding of fibers
• Simple pre-treatment and strong operability
• And should recommend to use 1.0um polypropylene membrane, 0.3um>99.95%

Some tips about using glass fiber filter membranes:

1. What should I do if the glass fiber membrane is broken during filtration?

• The reasons for the rupture of the filter membrane: one is that the material cannot withstand the dissolution of the sample solvent
• And the other is the pressure that the ultrafilter can withstand during filtration.
• Then the corresponding solution is to choose a filter membrane that is resistant to the sample solvent and apply the appropriate pressure
• And especially when using a filter with a smaller diameter (for the pressure resistance of the filter, please refer to the corresponding COA).

2. What should I do if the flow rate is too slow to affect the efficiency when filtering the sample?

• When the flow rate is too slow, we need to determine whether the sample contains high particulate matter and whether the sample viscosity is too high.
• In this case, high-speed centrifugation can be performed to remove particulate matter
• And an appropriate solvent can be selected for dilution, or a filter with a larger diameter can be used.

3. What is the difference between glass fiber filter paper and conventional nylon and other microporous membranes?

• From the perspective of filtration mechanism, glass fiber filter paper is a deep filter, which is characterized by trapping particles on the surface and in the medium matrix at the same time.
• It has the characteristics of deep filtration, fast flow rate, and large loading capacity;
• While the thickness of microporous filtration membranes such as nylon is relatively thin, and it tends to surface filtration.