2020年5月21日星期四

310rpm Oil & Water Bath, Color/Touch Screen Rotary Evaporator

This new type large LCD screen 
rotating evaporator is developed based on the demand of global laboratory users.
To
 

buy rotary evaporator

 
, RE1-OW-T Series will be the most advanced choice for you:
1. The big vivid LCD screen looks fancy to use.
2. Wide temperature range (RT-180/210°C) for both water and oil bath.
3. Fastest rotating speed (310rmp/min) for most experiments.
4. Triple-layered coil condenser equipped with the dripping point increases condensation efficiency.
5. Positive and negative rotate function designed for drying of powder.
6. And the safest protection function of auto-lifting in over-temperature or outage, makes Hawach RE1-OW-T Series rotating evaporator the most reliable to buy.
Features of RE1-OW-T Series rotating evaporator

• The rotating speed can reach 310rmp/min for crystalization experiment.
• The temperature of the rotating evaporator can reach 180/210°C for both oil and water baths, which is controlled by PID with Temp. The precision of ±2°C.
• Big LCD touch screen displaying both temperature and rotating speed.
• Effective 3-layered condenser and dripping point design prevent backflow of evaporation and increased evaporation process.
• Auto-lifting design in overheat or stop prevents the rotating bottle from breakage; Integral bath with a safety handle which is safe and easy to clean.
• Fast rotating speed and big temperature range provides more options for evaporation of most materials.

Double-Stage Stainless Steel Molecular Distillation

Molecular distillation is different from general conventional distillation, which does not reach gas-liquid equilibrium. The separation of molecular distillation is based on the different volatilities of different substances, and the separation operation is carried out below the boiling point of materials. The process is:
• Molecular diffusion from the main liquid phase to the evaporating surface. The diffusion rate in the liquid phase is the main factor to control the molecular distillation rate;
• The molecules evaporate freely from the evaporation surface, and the evaporation happens at a temperature far below the boiling point in a high vacuum;
• Molecules flying from evaporation to condensation, under the suitable vacuum, as long as the average free path of the evaporated molecule is greater than or equal to the distance between the evaporated surface and the condensed surface;
• The molecule condenses on the condensing surface, thus completing the separation and extraction of the substance molecule;
• Distillate and residue collection.
Key parameter condition of stainless steel wiped film distillation
• The faster the feeding speed is, the more heavy components will affect the evaporation
• The faster the mixing speed is, the thinner the film is formed and the longer the residence time is, but it will also hinder the number of light molecules moving to the condenser tube
• The higher the temperature of stainless steel wiped thin-film evaporator, the better the evaporation effect, but it also affects the separation rate
• The internal cooling temperature needs to be higher than the melting point temperature of the product
• Under the vacuum, it can protect the stability of products and change the boiling point of materials. The higher the vacuum degree, the better the effect
• Feeding temperature is closer to the temperature of 

stainless steel wiped thin film evaporator

, it is easier to evaporate and fractionate.
The structure of stainless steel wiped film distillation for sale
A complete set of double stage 
stainless steel molecular distillation for sale
includes: feeding system + main evaporator system + heating system + cooling system + vacuum system +material collection part
The characteristic of Hawach scientific stainless Steel molecular distillation for sale
1. The jacketed feeding tank is designed to preheat the materials and speed up the rectification efficiency; the built-in filter screen can pre-filter the materials and keep the purity of the materials;
2. Gear pump continuous feeding and discharging system, with glass eyepiece design of Hawach 
stainless steel wiped film distillation at the collection place, which can observe the feeding and discharging conditions;
3. Double-layer insulation pipe design, to maintain the stability of material temperature and ensure the continuous fluidity of materials;
4. The whole double stage stainless molecular distillation unit is made of stainless steel. The part contacting with the material is made of 316. The other parts are made of food-grade 304 stainless steel, which is durable and corrosion-resistant;
5.
Double stage stainless steel molecular distillation adopts frequency conversion motor to keep the evaporator rotor rotating stably;
stainless steel wiped thin film evaporator

6. The stainless steel wiped thin film evaporator has a built-in high-efficiency coil condenser, which is vertically parallel to the rotor, and the light components can condense immediately when contacting the condensing surface;
7. Standard with ball milling glass collecting bottle, optional with stainless steel collecting tank;
8. The external process coil condenser is used in combination with the waste cold well condenser to prevent the waste gas from entering the vacuum pump and causing damage;
9. The main evaporator of double stage stainless molecular distillation adopts magnetic seal with good sealing effect; the discharge port is equipped with imported three-piece vacuum ball valve; other parts are sealed with flange and PTFE / fluoro rubber, and the no-load leakage rate of the equipment is less than 0.1pa/5s;
10. Double stage stainless molecular distillation is designed with vacuum meter and regulating valve, accurate vacuum degree display, providing an effective reference for vacuum regulation or equipment maintenance;
11. The integrated control cabinet enhances the automation level of double stage stainless steel molecular distillation equipment.

Basic Instructions For Vacuum Filtration

Vacuum filter is the equipment for solid and liquid separation, which is formed by the pressure difference between the upstream and downstream sides of the filtration medium. The commonly used vacuum degree of a vacuum filter is 0.05~0.08MPa, but there are more than 0.09MPa. Vacuum filters can be intermittent and continuous two types. The continuous vacuum filter and the continuous vacuum filter have their own characteristics, but the continuous vacuum filter is more widely used. The common continuous vacuum filter has a drum type and rotary disc type.
Stainless Steel Three-Branches Vacuum Filtrations
The advantages of vacuum filter are as follows:
(1) Small labor intensity;
(2) Can directly observe the filtering situation, timely find out the problem, easy to check;
(3) Maintenance costs are low;
(4) The continuous vacuum filter has high working efficiency.
The disadvantages of vacuum filter are as follows:
(1) Cannot filter the material with low boiling point filtrate;
(2) The vacuum system needs regular maintenance;
(3) If the solid particle concentration and particle size distribution in the feed slurry change greatly in the filtration process of continuous vacuum filter, the filtration effect is poor.
Rotary vacuum filter
Its main body is a rotating horizontal cylinder, called a rotating drum, with a diameter of 0.3~4.5m and a length of 3~ 6m. The outer surface of the cylinder is composed of a porous plate or a special drainage member covered with a press cloth. The inside of the cylinder is divided into a number of fan-shaped compartments, each of which has a straw connected with a hole in the hollow shaft, and the hole in the hollow shaft leads axially to a rotating disk located at the end of the shaft and rotating with the shaft.
The rotating disc fits closely with the fixing disc to form a special rotating valve called the distribution head, as shown in figure (c) on the right. The fixed disk of the distribution head is divided into several arc-shaped voids, which are connected with the pressure relief tube, the washing liquid storage tank, and the compressed air pipeline respectively.
When the drum rotates, the fan can obtain vacuum and pressure respectively by using the function of the distribution head, so that the filtration, washing, and other operations can be controlled step by step.
Advantages of rotary vacuum filter:
(1) Continuous production, the higher degree of mechanization;
(2) According to the nature of the material, process requirements, using different materials to manufacture various types, in order to meet different filtration requirements (usually, for the suspension of medium particle size, viscosity is not too big material, rotary drum vacuum filter is applicable);
(3) Filter cake thickness and washing effect can be controlled by adjusting rotating drum speed; The loss of press cloth is smaller than that of other types of filters.
Disadvantages of rotary drum vacuum filter:
(1) Filter force is small, it is only the use of vacuum as the driving force. Due to the pipeline resistance loss, the maximum is no more than 80kPa, generally 26.7-66.7kpa, so it is not easy to drain, the final humidity of the filter cake is generally more than 20%;
(2) Equipment processing and manufacturing complex, the main equipment and auxiliary vacuum equipment investment is expensive, high energy consumption in the vacuum, at the same time, the larger the filter area manufacturing more difficult. The maximum filtration area of domestic production is about 50 square meters, generally 5 ~ 40 square meters.
Rotary vacuum filter
And rotating with the axis of the filter disk (or rotary disk). The structure and operating principle of the rotary disk vacuum filter are similar to that of a rotary drum vacuum filter. Each sector of the disk has its own outlet pipe leading to the central axis, and when a number of disks are joined together, the outlet of the sector of a rotary disk forms a continuous channel with the corresponding outlet of the other rotary plates in the same phase Angle.
Similar to a rotary drum vacuum filter, these continuous channels are connected to a shaft end rotary valve (distribution head). The operation of the rotary disk vacuum filter is very similar to that of a rotary drum vacuum filter. Each turntable is equivalent to a drum, and the operating cycle is also controlled by a rotary valve. Each rotary table has its own filter cake unloading device, but unloading is more difficult.
Rotary vacuum filter machine has a very large filtration area, can be as large as 85 square meters, its unit filtration area covers less land, a press cloth is easy to replace, less consumption, energy consumption is low. Its disadvantage is poor washing of filter cake, washing water and suspension are easy to mix in the filter tank.
The basic flow
Vacuum filter is at the side of the filter medium caused a certain degree of negative pressure (vacuum) and make the filtrate discharge of solid-liquid separation, thus the driving force is small, generally from 0.04 to 0.06 MPa, in some cases, up to 0.08 MPa, due to the low pressure drop on either side of the filter cake, thus filtering speed slower, superfine material of the filter cake moisture content is higher, the vacuum filter is one of its main deficiencies; However, its advantage is that it can operate continuously under relatively simple mechanical conditions, and in most cases can obtain satisfactory performance indicators.
Therefore, compared with other types of filters (such as filter press), the vacuum filter has been favored by users for a long time. Unless the filter press achieves a major breakthrough in continuous operation, mechanical properties, manufacturing, and operating costs, and can meet the comprehensive requirements of the application sector, vacuum filtration will be difficult to shake its important position in the solid-liquid separation field.
500ml-Glass-Solvent-Filter-for-Lab
The working cycle of the vacuum filter can be generally divided into the following stages:
(1) Dehydration stage;
(2) Washing stage,
(3) Compaction stage;
(4) Drying stage;
(5) Cake unloading stage. Among them, washing, compaction, drying, and other stages are determined in disregard of the actual needs, while cake making, dehydration, and cake unloading are the basic working processes of most vacuum filters (in the filtration cycle of horizontal belt vacuum filters, the cake unloading stage can be excluded).In the filtering cycle, the timeshare of each operation varies with the filter.
Filter cake formation stage strictly processes (filter in terms of its significance is refers to the filtrate through continuous media the process of the separation of the solid materials), in the process of solid material by vacuum effect, lower feed or vacuum and upper (feed) by the joint action of gravity and adsorption on the surface of the filter medium, gradually formed a certain thickness of the filter cake; With the gradual thickening of the filter cake, the corresponding filtration resistance also increases gradually, so the filtration speed (that is, the filtrate volume per unit area of filter media per unit time) in this stage gradually decreases.
However, the main task of this phase is to form a certain thickness of filter cake to achieve the desired processing capacity. After the filter cake is formed, it leaves the feed tank and enters the dehydration stage. In this stage, the relative saturation (that is, the ratio of the water volume of the filter cake to the total pore volume of the filter cake) of the filter cake decreases from the original 100% to 10%~20%. Under vacuum suction, most of the pores occupied by water are replaced by air.

Basic Instructions For Vacuum Filtration

2020年5月20日星期三

Comparison Of Flash Column Scheme And Initial Distillation Scheme

1. There are several schemes in the process design of atmospheric and vacuum distillation unit:
(1) Conventional electric desalting → atmospheric tower → vacuum tower process.
(2) Electric desalting → flash tower → atmospheric tower → vacuum tower process.
(3) Electric desalting → primary distillation tower → atmospheric tower → vacuum tower process.
In order to recover light hydrocarbon, after the atmospheric and vacuum distillation process, the process is divided into compressor scheme and primary distillation tower pressurization scheme (without compressor scheme).
2. Scheme comparison
The first is the pressurization scheme of the primary distillation tower, which is the process of electric desalting → primary distillation tower → atmospheric tower → stabilizer, and the operating pressure of the primary distillation tower is 0.2MPa, so the compressor in front of the stabilizer is canceled.

The second is the flash column scheme, which is set with the flash column, that is, the process of electric desalting → flash column → atmospheric tower → pressure reducing tower → stabilizer. The flash tower is operated under atmospheric pressure, and a compressor is set in front of the stabilizer.
In order to increase the adaptability and flexibility of the unit, especially the adaptability of the unit to sulfur-containing light oil, there are basically two options for the atmospheric and vacuum distillation process. One is to use the pressure lifting scheme of the primary distillation tower to make the light hydrocarbon in the crude oil dissolve in the primary top oil as much as possible under the condition of slight pressure. The primary top oil is pumped to the stabilization tower to recover the light hydrocarbon.
The advantage of this scheme is that there is no compressor in the whole process, which reduces the amount of mechanical maintenance, but this scheme also has its disadvantages.
For example, a small part of light hydrocarbon will be taken to the flash column to lose from the overhead gas, and a complete set of reflux cooling system will be added to the initial top, which makes the process more complex.
Second, the flash tower is adopted. In this scheme, the light components of crude oil flash out of the flash tower and enter a suitable part of the atmospheric tower, making the heat exchange of flash bottom oil more reasonable, reducing the material flow into the atmospheric furnace to save energy, adding a compressor on the top of the atmospheric tower to compress and boost the overhead non condensable gas, and the overhead oil uses the pump to boost and the overhead gas after boosting to recover light hydrocarbon in the stabilization tower, Due to high pressure, the non condensable gas on the top of the stabilizer can be sent to the desulfurization system of the whole plant for desulfurization treatment.
The advantage of this scheme is that the flash tower is adopted, the process is simple, the non condensable gas of the unit can be desulfurized, and the environment can be protected. The disadvantage of this scheme is that the compressor needs to be set, and the maintenance is a little difficult. It is understood that at present, most of the foreign processing of high sulfur light crude oil adopts flash tank and constant top gas compressor scheme. The comparison of this project’s plans focuses on the advantages and disadvantages of these two plans.

Selection Of Purification Reagents For QuEChERS Method

About HAWACH and QuEChERS method
HAWACH aims to facilitate the lab experiment process and making it easier and simpler. Its extraction kits with 50 ml centrifuge tube and salt pack, purification kits with 2ml/15ml centrifuge tube that contains purification adsorbent, and 2ml/15ml/50ml ceramic proton are widely used in different labs all over the world. The QuEChERS method has been widely used in many aspects of drug residue detection since its inception, from the initial detection of pesticide residues in fruits and vegetables to the detection of veterinary drug residues in aquatic products, the detection of mycotoxins in cereals, the detection of illegal additives in food, etc. The wide variety, the different properties, and the complex composition of the sample matrix increase the detection difficulty to a certain extent. The QuEChERS method or the improved QuEChERS method has a wide range of detection in industrial dyes, alkaloids, illegal addition of drugs, synthetic antioxidants, etc.
Extraction reagent selection
Because acetonitrile has the advantages of easy salting out, compatibility with liquid chromatography instruments, and the ability to precipitate proteins, the extraction solution often selected by traditional QuEChERS methods is acetonitrile or acidified acetonitrile. However, the illegal additives in food itself contain functional groups and possess different properties. Adjust the extraction solvent to make the target more easily extracted is a problem to be considered.
QuEChERS D-SPE Kits
In addition, when using the QuEChERS method, a suitable amount of water is generally added to the dry sample (water content <25%) to ensure the extraction efficiency. Some researchers have tested the samples such as chili powder, chili paste, tofu skin, etc. adding water soaking samples. However, some researchers also directly added organic solvent extracts to the yeast and yeast extracts, without adding water for immersion. Therefore, whether to use water to destroy the target and the sample matrix before adding the extraction solution is also a factor to be considered.
Purification reagent selection
The matrix composition of some samples is too complex, and the QuEChERS method will produce a serious matrix effect. In addition to the use of methods such as reducing the sample volume, adding a blank matrix to prepare the standard, adding internal standard correction, etc., select efficient purification reagents in the purification is also one of the ways to eliminate matrix effects.
C18, GCB, PSA, and other purification reagents used in the traditional QuEChERS method have been unable to meet the needs of the detection of the addition of illegal substances in food. On the basis of ensuring high recovery, low detection limit, good repeatability, high precision, etc., develop new purification reagents are also the direction that can be developed in the future.
Combined with other instruments
In the current detection of illegally added substances in food, most researchers choose the test mode combining the QuEChERS method and liquid chromatography / liquid chromatography-tandem mass spectrometer. Because liquid chromatography-high resolution mass spectrometry technology has the advantages of accurate qualitative, non-directional unknown screening, high-throughput analysis, minimizing false positives, and the false-negative rate is close to zero, etc. Its applications are becoming more and more popular, making the combination with  QuEChERS become possible. Combining the advantages of the two, it can be foreseen that the sample processing process for the detection of illegally added substances in food in the future is simple, efficient, and accurate.
After continuous updating and improvement, the application of the QuEChERS method in the detection of illegally added substances in food will reduce the cost of conventional testing and improve the efficiency of testing that puzzles researchers.

2020年5月19日星期二

Use Of HPLC Column

Basic structure: chromatographic column including HPLC column tube, pressure cap/sleeve, the screen plate (filter), joint, protection column, protection column sleeve, protection column core.
Analytical
4mm conventional analysis HPLC column, 3.9mm conventional analysis column, 4.6mm conventional analysis column, 1.0mm narrow diameter column, 2.1mm narrow diameter column, 0.2-0.5mm capillary column.
The preparation of type
Semi-prepared column (5mm, 7.8mm, 9.8mm, 10mm), prepared column (21.2mm, 30mm, 50mm).
Packing substrate
1. Silica gel matrix – pH 2-8 — at high or low pH, silica gel dissolves
2. Al2O3·nH2O — ph1-14 — chemical modification is difficult
Polymer matrix – ph1-14 — the pore structure is complex, the pore size is not uniform, the column effect is not high enough, the organic solvent may cause the polymer matrix swelling and damage.
XChroma HPLC Column
Affecting Factors
A: physical factors
1. Silica gel purity — the purity and residual metal ion concentration of silica gel filler.
2. Column size — packed bed length and inside diameter.
3. Particle shape — irregular shape and round shape.
4. Particle size — average particle diameter, usually 3-10 m.
5. Surface area — the sum of the outside surface and the inside surface of the particle, expressed as m2/gram.
6. Aperture — the average size of a particle’s hole or cavity, range 80-300a.
B: chemical properties
1. Bond type — monomer bond — bond phase molecule is connected to the matrix at a single point.
2. Carbon coverage — the amount of bonding phase connected to the substrate.
3. End seal — after the bonding step, use a short chain to seal the exposed silica hydroxyl group.
Before choosing the chromatographic column, you should know more about your samples and impurities, their types, structures, polarity, acids and bases, molecular weight, etc.
1. The sample is polar and slightly acidic; Therefore, C18 HPLC column can be selected for detection in 100% acidic aqueous solution, that is, it should be selected to withstand 100% pure water and retain a good chromatographic column for polar compounds.
2. If the sample polarity is too strong, or the acidity is too strong, can choose CN, NH2, or silica gel column, HILIC (hydrophilic chromatography), there are also using C18 + strong anion of reagents or strong anion exchange chromatography column (defect is ion pair reagent balance time is long, the mobile phase pH requirements more precise, whether it is hard to repeat the experiment, it is difficult to wash down other ion-pairing reagent makes it, basically, with ion pair chromatography column) cannot be used for other experiments.
3. If the sample is alkaline; Can choose high purity silica gel column (high purity silicon lack of metal impurity, and silica gel base sealing) or a modified C18 column some (such as polarity or alkali to live embedded technology, etc.), they will reduce the trailing alkaline compound, usually choose neutral or alkali condition, because it can increase the alkaline reserved sample.
4. If the polarity of alkaline compounds is too strong, or too basic; Can choose wide pH C18 chromatographic column at high pH value detection (advantage is simple method development, the disadvantage is that be difficult to implement this technology at present, the price is high also) or choose HILIC chromatographic column (silica gel column under the condition of reverse phase is used, it is also a method to detect alkaline samples of the classic) choose strong ion exchange column, the disadvantage is that can’t be used to analyze other samples, the mobile phase pH requirements more precise, it’s hard to repeat the experiment) are using C18 + strong anion of reagents or strong anion exchange chromatography column.
How does the presence of bubbles in column chromatography affect the separation of samples? If there is a bubble, will reduce the separation ability, because the presence of the bubble phase to reduce the action site, reduce the force between the packing and the material. Therefore, in order to avoid the occurrence of this situation, when loading the tomographic column, the column wall should be continuously knocked and interference should be avoided.

C8 SPE Cartridge And Comparison Among Flash Column And HPLC Column

This matrix is a silica gel particle with a certain pore size and specific surface area, which is modified by bonding or other chemical or physical means to make the silica gel surface and provides some chromatographic properties. This kind of filler has the advantages of mature technology, high quality, and stability, wide application range, economical and durable, etc. The deficiency is poor alkali resistance.
This type of filler requires high purity, water-soluble or oil-soluble impurities. For some large molecular samples with complex structures, such samples, it has unique effects. Moreover, these kinds of fillers are stabilized packing, there will be no common problems such as filler loose in the operation process.
There are many features of the C8 SPE cartridge. For example, it is extremely suitable for purification of the strongly retained substances. Also, the polar interaction is not the critical feature of the C8 SPE cartridge, its retention of the solid phase is relevantly weaker than the C18 SPE cartridge.
Reversed Phase C18 SPE Cartridges
Moreover, although the C8 SPE cartridge is similar in properties with the C18 SPE cartridge, as the former one has shorter hydrocarbon chain and lower carbon loadings, its retention capacity of the nonpolar chemical compound is weaker than the latter one. When the analyte retains too strongly to be effectively eluted in the C18 SPE cartridge, the C8 SPE cartridge is a good substitute. However, as the carbon chain of the C18 SPE cartridge is shorter and the coverage of the silica gel surface is smaller, its potential polar interaction with the analyte is a litter stronger than the C18 SPE cartridge. Once again, the polar interaction is not an important feature of the C8 SPE cartridge.
On the whole, the substances that are most commonly used as the matrix of the C8 SPE cartridge are liquid samples and biological fluids. And, the main extraction mechanism of the C8 SPE cartridge is non-polarity.
SPE and Flash column
Both flash columns and SPE are to separate in the sample preparation. The difference is that the flash column is a fast separation column, which is used with a certain instrument. The upper interface generally has an air/liquid (with a certain pressure) to speed up the sample separation process during usage. SPE mostly uses gravity to flow down by itself or with SPE equipment to speed up the separation by vacuuming. Flash columns are commonly used in organic synthesis and industrial use (industrial preparation will also need large columns with heavy packing); SPE is usually seen at small sample pretreatment in the laboratory.
SPE, flash column and HPLC column
In general, SPE and FLASH are used in the pre-treatment of the sample to be analyzed. It can be understood as separating the salt from the sugar and obtaining a pre-treatment solution of the sugar. The HPLC column was then used to separate glucose, maltose, and sucrose from the sugars in an HPLC instrument. It should be noted here that the properties of salt and sugar are very different, so they are well separated, but glucose, maltose, and sucrose in sugar are very similar in nature. Just like triple sisters, it is more difficult to separate. Therefore, the separation capacity of the HPLC column is required to be much larger than that of SPE or FLASH. This is why the HPLC column is more demanding and expensive.

C8 SPE Cartridge And Comparison Among Flash Column And HPLC Column