-50°C Freeze Dryer; Digital Display

 Freeze Dryer -50 Series is a small freeze dryer, it can be divided into ordinary type, gland type, ordinary bottle hanging type, gland bottle hanging type and T rack multi-manifold type. According to the different types, we have 5 types freeze dryer for sale. What the freeze dryer for sale is our common type.

If you want to customize, we also can support you. Depending on the nature of the test sample, We will recommend the suitable one freeze dryer for sale.

Due to the small freeze dryer, it is easy to operate and move. Compared with the vertical freeze dryer lab equipment, the lyophilization machine price has obvious advantages and is widely welcomed by the laboratory. Due to the compact design of the desktop lyophilizer, the floor area is relatively small and the appearance is beautiful, the lyophilized area is not large, generally 0.1-0.12 square meters, equipped with a 4-layer material tray, which fully meets the laboratory’s small experiments.

The temperature of the cold trap is generally -50 degrees, using stainless steel, no coils inside, large openings, smooth and corrosion-resistant, which can reduce material pollution.

Freeze dryer -50 series tray lyophilizer with pre-freezing function, digital display temperature, and vacuum. The imported closed compressor is highly efficient and reliable, with low noise and excellent performance. At the same time, it has the originally imported inflation valve, which can be filled with dry nitrogen or inert gas.

The Freeze Dryer -50 Series tray lyophilizer is equipped with a patented diversion tube to speed up the lyophilization process and greatly reduce the experimental time. In order to better observe the experiment, a transparent bell-type drying chamber is used, which is safe and intuitive. According to the needs of different customers, Freeze Dryer -50 Series tray lyophilizer is also available with sample temperature display and -80 degrees.

Advantages of small freeze dryer method over traditional drying

Methods for drying are various, sun-drying, cooking, spray-drying, vacuum drying, etc. However, ordinary drying methods are usually performed at a temperature of 0°C or higher. Generally, the products obtained by drying have the problems of shrinking in size and hardening the texture. Most of the volatile components will be lost, some heat-sensitive substances will be denatured and deactivated, and some substances will even be oxidized. Therefore, compared with the product before drying, the properties of the product after drying are very different.

The lyophilization rule is basically carried out below 0°C, that is, in a frozen state of the product, and generally does not exceed 60°C when analyzing and drying. Under vacuum conditions, when water vapor directly sublimates, the drug remains in the ice shelf when it freezes, forming a sponge-like loose porous structure, so its volume will not change after drying. Before reuse, as long as water for injection is added, it will immediately dissolve, maintaining the original structure, and no concentration phenomenon will occur.

-50°C Freeze Dryer; Digital Display

310rpm Rotary Vacuum Evaporator

In terms of display type and heating type, the RE1 series small rotovap can be classified into the RE1-OW-T series of oil&water bath and color LCD screen, RE1-OW-L series of oil&water bath and standard LCD screen, RE1-OW-L series of water bath and standard LCD screen.

Compared with rotary evaporator parts and large scale rotovap, the speed of rotary vacuum evaporator is the largest, reaching 310rpm. Each series is available in 2L, 3L, and 5L rotovap size with the following specifications:

1. RE1-OW-T series tabletop rotovap: 310rpm; 210℃ oil&water bath; LCD touch screen or color LCD screen; 3-layered condenser; auto lifting protection rotary vacuum evaporator with positive&negative rotating function

2. RE1-OW-L series rotary vacuum evaporator is with rotary speed of max. 310rpm; heating temperature of max.180°C for both Oil&Water Bath; standard LCD Screen; Dual-reflux coil condenser; and Autolifting protection with positive & negative rotating function for powder drying.

Interface between the condenser and receiving flask: A type ball mill mouth(closely sealed, more popularly used in small rotovap) and B type standard mouth to choose.

3. RE1-W-L series small rotovap: 310rpm maximum; max. 99°C temperature for Water Bath; LCD Screen display rotating speed and temperature; Dual-reflux coil condenser with big condensation area; Autolifting in over temperature or abnormal substance with positive & negative rotating to dry powder products.

Regarding different condenser and rotary flask, RE1-W-L series rotary evaporators can be classified into 3 groups: standard vertical condenser type rotary vacuum evaporator, horizontal condenser type tabletop rotovap and 5 in 1 rotating bottle type small rotovap, with details listed as follows.

310rpm Rotary Vacuum Evaporator

Development of Flash Column Method

 The flash column method is an alternative to long column chromatography developed in 1978.

The method aims to separate the components from the mixture, thereby purifying it. It is established from the existing long-column chromatography technology, which is time-consuming and often unsatisfactory. In short, the flash column method allows the sample to pass through a column filled with gel, and the gel separates the sample.

Still, the founder of the flash column and his colleagues have been using medium pressure chromatography and short column chromatography to replace long column chromatography. They decided to combine the two to overcome the shortcomings of long column chromatography, which is time-consuming and low recovery.

The gel originally used to pack the column was silica gel, which is still widely used. They used air pressure to push the solvent through the silica column, compressing the column. The sample is then applied and the same (can be different, but usually the same) solvent is used to pass the sample through the column. Then collect the purified components or fractions, the whole process takes about 5-10 minutes. Usually, a small part appears first and most eluted last. The collected fractions were subjected to original analysis by thin layer chromatography (TLC) plates.

Since its introduction, the silica gel flash column has been widely used in organic chemistry. However, guidelines are often loose, driven by personal experience, or not translated well when settings are changed. However, some things are still true. Increasing the sample size will cause the resolution to decrease.

Compared with the HPLC column, the resolution of the flash column is already at an intermediate level, but it is sufficient for sufficient separation, so the increase in the number will only worsen the situation. Second, the optimal flow rate depends on the length and width of the column and the nature of the gel.

This is due to the number of plates available, for example, longer and narrower columns will provide more theoretical plates, thereby affecting the flow rate. Finally, the resolution is affected by the stationary phase. If the stationary phase or gel arranged on the column is more uniform and has a smaller particle size, it provides better resolution. The smaller the particle size, the larger the surface area, and the higher the resolution.

Manipulating all these factors to optimize the purity or recovery of the components can be quite complicated because they interact, but they have different effects when tested independently. For example, the mobile phase selectivity has the greatest impact on the resolution, but it depends on the column capacity. Then, this will be affected by the chosen solvent.

Therefore, before starting any real experiment, you need to test or calculate the settings used in the flash column. If you have data from TLC, you can calculate the best settings without testing any flash column settings. Low TLC delay factor (Rf) provides better separation. Using such data, the amount of solvent required can be calculated because they are inversely proportional to each other, and both are related to the column void volume (the solvent in the column before the sample is loaded).

In the original method, it was very important to prevent the column from drying out. However, a more user-friendly alternative has since been developed, called dry column flash chromatography. This method has been adopted by first-time students, while still producing good results comparable to the quality of TLC analysis.

The principle is the same, but the column contains dry silica gel. The powdered xerogel is filled into the column by suction, and finally, a uniform bed of about 1 cm for solvent and sample is obtained. The column was also eluted by suction and dried after each fraction.

Development of Flash Column Method

Boxed Sterile Filter Tips

Introduction of low retention tips

Hawach pipette tips are universal fit, the tips can be used with most of the brands in single-channel pipette and multi-channel pipette. such as Eppendorf, Gilson, Rainin, Biohit, Brand, Finpipette, etc. Hawach pipette tips are available in three different packages, respectively are bags, racks, and stacked packages. Bag packaging is the most cost-effective option; Rack/box packaging is convenient to use and stock; Stack packaging is economic for tips stock management.

Features

1. Electron Beam Sterilization, this way make sure the sterile tips with no chemical residue after sterilization, no interference for virus detection
2. Non-pyrogenic, Non-endotoxin, DNase/RNase free
3. 100,000 class clean room for production and packaging
4. Universal fit with most pipettes on the market
5. Super fine grinding molding technology which does not need agent demolding ensures smooth inner wall of low retention tips with minimum liquid residue and precise liquid absorption
6. Pipette tips made of virgin PP with high transparency and wide chemical resistance
7. PE sintered filter as a barrier to against aerosol, which eliminates the risk of cross-contamination between the sample and the pipette
8. Filtered pipette tips can be used for suck up of organic solvent products
9. Optimized pore size: ensures smooth absorption of sterile tips. Innovative design, which ensures good flexibility, good sealing, and compatibility of the filtered pipette tip
10. Wide size range: size 10ul, 10ul extended, 200ul, 300ul, 1000ul, 1250ul. Available in standard and low retention tips
11. Wide packaging range of sterile tips: bags, rack, and stack to choose
Bag packaging is the most cost-effective option
Innovative design and special molding technology, which ensures precise and smooth adsorption of low retention tips
Special electric beam sterilization technology ensures the sterility property of our pipette tip box
12. OEM service available

Comparison between tips and filter tips

Tips
Filtered pipette tips

Comparison between standard tips and low retention tips

	Standard tips	Low adsorption tips

Tips in different packaging:

1000pcs/bag
96-100pcs pipette tip box
96pcs/stack

Technical Parameters

Standard tips and low retention tips:

Picture	Model NO.	Size	Description	pcs/carton	G.W (kg)	Carton Size (cm)
Bulk Package
	T-10-D	10ul	Standard tips 1000pcs*20bags/carton	20000	3.0	44*26*19
	T-10-D-L	10ul long	Standard tips 1000pcs*20bags/carton	20000	3.0	44*26*19
	T-20-D	20ul	Standard tips 1000pcs*20bags/carton	20000	7.0	60*32*30
	T-100-D	100ul	Standard tips 1000pcs*20bags/carton	20000	7.0	60*32*30
	T-200-D	200ul	Standard tips 1000pcs*20bags/carton	20000	7.0	60*32*30
	T-300-D	300ul/200ul long	Standard tips 1000pcs*20bags/carton	20000	7.0	60*32*30
	T-1000-D	1000ul	Standard tips 1000pcs*5bags/carton	5000	6.0	70*25*34
	T-1250-D	1250ul/1000ul long	Standard tips 1000pcs*5bags/carton	5000	6.0	70*25*34
Rack Package
	T-10-HS	10ul	Sterile tips 96pcs*50boxes/carton	4800	7.0	50*35*29
	T-10-HS-L	10ul long	Sterile tips 96pcs*50boxes/carton	4800	7.0	50*35*29
	T-20-HS	20ul	Sterile tips 96pcs*50boxes/carton	4800	8.5	49*40*28
	T-100-HS	100ul	Sterile tips 96pcs*50boxes/carton	4800	8.5	49*40*28
	T-200-HS	200ul	Sterile tips 96pcs*50boxes/carton	4800	8.5	49*40*28
	T-300-HS	300ul/200ul long	Sterile tips 96pcs*50boxes/carton	4800	8.5	49*40*28
	T-1000-HS	1000ul	Sterile tips 100pcs*50boxes/carton	5000	13.0	60*52*29
	T-1250-HS	1250ul/1000ul long	Sterile tips 96pcs*50boxes/carton	4800	14.0	60*49*28
Stack Package
	T-10-RHS	10ul	Sterile tips 96pcs*50stacks/carton	4800	14.0	45.5*24*26.5
	T-200-RHS	200ul	Sterile tips 96pcs*50stacks/carton	4800	18.0	45*29*26.5
	T-1250-RHS	1250ul/1000ul long	Sterile tips 96pcs*50stacks/carton	4800	28.0	43*28*27

Filtered pipette tips:

Boxed Sterile Filter Tips

Some Introduction of Vacuum Filtration

 Hawach vacuum filtrations are faster than simple gravity filtrations with a simple conical funnel. In order to obtain a better performance, there are some points that need to be considered to win the application of vacuum filtration. Reduced pressure and special attention have paid to the equipment used in this procedure.

The glassware should not occur any cracks on the bottoms or the sides. It is also significant that the vacuum trap and the filter flask are clamped accurately, in this way, they will not move during the filtration process. The thick-walled tubing acts as a whip, and the filter flask is slammed into the metal objects resulting in an implosion.

Solvent Particle Removal of Glass Solvent Filter

Hawach glass solvent filter is designed for solvent particulate removal and aqueous mobile phase filtration. All parts of glass solvent filter contacting with solvents are made of chemically inert glass or PTFE material, which can be used in conjunction with regenerated cellulose (RC) filter membrane, especially suitable for particle removal and degassing filtration of the mobile phase during chemical analysis such as HPLC、GC、AA.

The outlet position is far lower than the vacuum pump interface position, which makes the filtration process safer. A hose made of polypropylene is used to safely connect the vacuum hose.

Reliable Quality and Standard Specification

Hawach selects high-quality glass material to make glass solvent filter, so the wall thickness is uniform, no bubble, the glass is smooth and transparent, the flow rate is fast, the pressure resistance and sealing performance are very good, the size specification conforms to the international standard, these properties make it can be used for high temperature and high-pressure sterilization.

A completely clean vacuum environment is provided, and the inner side of the glass solvent filter does not require maintenance and does not produce any contaminants.

The function of Vacuum Pump

Hawach vacuum pump is a device that uses mechanical, physical, or chemical methods to obtain a vacuum. Generally speaking, a vacuum pump is a device to improve, produce, and maintain a vacuum in a closed space by various methods.

If the material contains water or organic solvent, add liquid water filter and condenser to play a filtering role. While using a vacuum dryer, we must strengthen the maintenance of the vacuum pump, change the oil to a vacuum pump in time, and prolong its service life.

Structure of Vacuum Filter

The overall structure of the pump is determined by the layout structure of the pump body. The vertical structure of the inlet and outlet horizontal setting, assembly, and connection lines are more convenient.

However, the center of gravity of the pump is high and the stability is poor at high speed, so this type is mostly used for small pumps. The air inlet of the horizontal pump is up and the exhaust port is down. Sometimes for the vacuum system pipe installation and the connection is convenient, the exhaust port can be connected from the horizontal direction, that is, the inlet and exhaust direction is perpendicular to each other.

Attention in Vacuum Filtration

In operation process, you should never perform a vacuum filtration without a vacuum trap and better keep the trap clean, or it is easy to get sucked in there.

Hawach vacuum filtration can be performed with funnels that have a flat surface. What we should pay attention to is that vacuum filtration is not fit for solvents with low boiling points such as dichloromethane, diethyl, or other similar solvents. Moreover, when you are done with the vacuum filtration, you should remove the tubing from the filter flask before the turn off the vacuum filtration.

Some Introduction of Vacuum Filtration

About The Features And Usages Of Glass Fiber Extraction Thimbles

HAWACH provides customers with two main kinds of extraction thimbles, cellulose, and glass fiber. Today's focus is on the glass fiber ones. The glass fiber extraction thimble belongs to the deep layer filtration, and its main purpose is to be used as the filter layer and directly added to the filter membrane. It is made of 100% glass fiber, with the characteristics of a high flow rate, strong dirt holding capacity, which can filter fine particles. Compared with other fiber materials and synthetic materials, glass fiber extraction thimble has higher filtration efficiency and higher dirt holding capacity.

Characteristics of glass fiber extraction thimbles
1. Made of glass fiber, without adhesive.
High temperature resistance: can withstand high temperatures of 500 ℃. However, the HAWACH glass fiber extraction thimbles’ maximum temperature can reach 600 ℃.
The large surface area makes the glass fiber filter paper have excellent retention capacity and a high flow rate.
Filtering difficult-to-filter substances or highly contaminated solutions can reduce the cost of filtration.

Usages
The HAWACH glass fiber extraction thimbles can be used in Soxhlet extraction: when the temperature is higher than 120℃, strong acid and other cellulose filter cartridges cannot be tried. Soxhlet extractor is used in the determination of crude fat content in commercial grains and oilseeds, whose three major components are heating extraction, solvent recovery, and cooling. The heating temperature of the Soxhlet extractor can be flexibly adjusted according to the ambient temperature during operation and the boiling point of the reagent.

In order to achieve the purpose of rapid extraction, the sample of the fat analyzer is soaked and extracted repeatedly in the extraction process. The solvent can be recovered automatically, which saves a lot of time and greatly facilitates the use of users.  The extraction time is adjustable, and an alarm will be issued when the time comes.

Also, the glass fiber extraction thimbles are to collect particulate matter such as smog in the atmosphere. The effect is better when used with smoke collection equipment. It is suitable for stationary pollution source detection: smoke, and dust, such as cadmium, nickel, tin, fluoride, etc. from fixed atmospheric pollution sources; Fixed pollution source waste gas benzoic ratio, lead, pitch smoke, sulfuric acid fog, etc.; Ambient air and exhaust arsenic, dioxins, etc.

Note: HAWACH glass fiber extraction thimbles have three sizes(SLGET32120, SLGET2590, and SLGET2870). When the diameter is too large, the edge will extend under the sealing ring and cause leakage. When filtering more viscous and difficult-to-filter liquids, such as serum, multi-layer filtration is usually used.

About The Features And Usages Of Glass Fiber Extraction Thimbles