显示标签为“pipette”的博文。显示所有博文
显示标签为“pipette”的博文。显示所有博文

2022年7月10日星期日

Brief Introductions of Pipettes

What are pipettes– A pipette is a measuring instrument with a certain range, which can quantitatively pipette or add liquid reagents, and has the characteristics of fast and accurate!

8 Channel Pipette single channel pipette

The predecessor of the pipette:

  • More than a hundred years ago, the pipette use to transfer liquids. The so-called pipette is a hollow glass tube marked with some scales.
  • Insert this glass tube into the liquid, and use the mouth on the other end of the tube or the ear ball to draw the liquid into the tube, the scale at the top tells us how much liquid there is.
  • After filling the amount we need, plug the end of the straw with your thumb, then place the tube into another container, release the handle, and let the liquid in the tube flow into the second container.
  • This completes our task of transferring the liquid!

The birth of the pipette:

  • However, with the progress of society, the elites found that the pipette was really difficult to use, tired and slow, and it was not easy to clean.
  • As a result, loose thoughts accumulate, accumulate, and explode in the brain – and the gorgeous pipette is born!

How A pipette work:

  • Generally speaking, there are two types of pipettes, one is the exhaust type (the official name, which is the pipette we usually use);
  • The other is the external piston-type often as a pipette, it is often for transferring special liquids, and the application range is relatively narrow.
  • The so-called exhaust type means that the piston is pressed down and the air inside the lower end of the pipette is pressed out.
  • Then, when the piston moves upward, the air pressure inside the lower end of the pipette is smaller than the external air pressure
  • The so-called outer piston type is actually the same as the principle of the syringe.
  • And if you have seen the working process of the syringe, you can probably understand the principle of the outer piston type.

Pipettes volume range:

  • The original pipette cannot adjust the volume, which fixed volume pipette on the market now.
  • Over time, this pipette has struggled to meet the needs of the modern researcher.
  • Later, a pipette with an adjustable range appeared, but there are fewer optional ranges, which is indeed much more convenient than the previous one.
  • However, this kind of pipette is limited in range selection and gradually eliminated. We also classify this type of pipette as a fixed volume pipette.
  • Freely adjusted within a certain range in the mainstream pipettes on the market, which are adjustable volume pipettes.
  • Generally speaking, the volume adjustment range of the pipette is 10%-100% of its marked larger volume.

The number of pipettes channels:

  • From the first pipette to the mainstream of the pipette market, only one transferred liquid sample at a time, which we call a single-channel pipette.
  • But with the rapid development of the life science field, many times single-channel pipette means inefficiency.
  • Thus, multi-channel pipettes have emerged, that is, a single pipetting operation can transfer multiple liquid samples.
  • There are many types of multi-channel pipettes on the market today, including 6 channels, 8 channels,  12 channel multichannel pipettes, 16 channels, 24 channels, and even 36 channels, 48 ​​channels, 64 channels, etc.
  • For a long time since accomplished the introduction of pipettes, aspiration, and discharge by the movement of a thumb-driven piston.
  • In short, an electric pipette uses a tiny motor to drive the piston movement, allowing people to aspirate and dispense liquid with the push of a button.
  • In this way, the thumb does not have to complain every day. We believe that the replacement of manual pipettes with electric pipettes is an irreversible trend in the future.

There are more introduces about what is adjustable pipettes, how to disinfect a pipette, etc.

2021年2月1日星期一

Calibrating Multichannel Pipettor To Prevent Errors

Calibrating Multichannel Pipettor To Prevent Errors

Widely used in chemistry, molecular biology research, and medical tests in the lab as well, a pipettor is a handy instrument for transporting a measured volume of liquid. It can draw up and dispense liquid by creating a vacuum above the liquid-holding chamber and releasing this vacuum. From single piece glass pipettors to complex adjustable or electronic pipettor, Hawach provides pipettors in several designs for various purposes, and in different levels of accuracy and precision too.

On one hand, a pipettor needs are accurate to the degree with the delivered volume being equal to the specified volume, on the other hand, for precision, it needs to be concerned with the closeness of several measurements to each other. For example, a pipettor might be consistently inaccurate but this inaccuracy could be very precise you still can get the reproducible result out of your samples.

Advanced-Fully-Autoclave-8-12-Channel-Adjustable-Pipettes

The scientists always prefer the perfect pipetting with accuracy and precision for their applications. It is important to work hard to get maxim accuracy and precision and reduce the level of uncertainty at the same time. To reduce the risk of error in manual liquid handling, you need to choose Hawach pipetting system, consisting of the highest quality pipettors professionally maintained and calibrated. And they can be used with the high quality matched tips. With Hawach pipetting products on hands, the correct pipetting angle, speed, and a smooth, consistent pipetting rhythm will help you get better results.

In order to prevent errors when using a multi-channel pipettor, you must learn how to calibrate the main characteristics of the multichannel pipettor:
1. Large-sized central pipetting control button, independent tip removal button.
2. Ergonomically designed finger rest that fits the hand and is easy to hold.
3. Real one-handed operation-regardless of the left or right hand, you can easily set the pipetting volume with one hand.
4. Range lock to prevent unintentional range changes.

Calibration steps for multichannel pipettors
a. Adjust the pipettor to the volume to be calibrated and select the appropriate tip;
b.Adjust the balance;
c. Suck and blow distilled water back and forth 3 times to make the tip wet, wipe the tip with gauze;
d. Hold the pipettor vertically, immerse the tip into the liquid surface 2 ~ 3mm, and slowly (1 ~ 3 seconds) suck the distilled water consistently;
e. Remove the tip from the liquid surface and lean against the tube wall to remove the liquid outside the tip;
f.Put the pipettor into the weighing beaker at an angle of 30 °C. Slowly and uniformly press the pipettor to the first position, wait for 1 to 3 seconds, and then press it to the second position to completely discharge the liquid in the tip;
g. Record the weighing value;
h. Dry the outside of the tip;
i. Weigh 10 times according to the above steps;
j.Take the average value of 10 measurements as the weight of distilled water absorbed by the final pipettor, and calculate the volume according to the Z factor of distilled water listed in Appendix 1;
k. Adjust the pipettor according to the calibration result.
The above is all about sharing how to calibrate in order to prevent mistakes when using multi-channel pipettors today. I hope it will be helpful for everyone to use this device in the future.

Calibrating Multichannel Pipettor To Prevent Errors