Developed in the 1970s, flash column chromatography is the method of isolating and purifying a component from a mixture by passing the sample through the flash column filled with a gel.
The inventors of flash column chromatography were using medium pressure chromatography and short column chromatography to replace the long column chromatography. And then they found a new way of combining the pressure and short column together to avoid taking too much time and achieving poor recovery.
The technique of flash column chromatography is well known because it presents the faster flow rates of the solvent than simple gravity flow. The silica gel was used as the original gel to line the column. And the silica gel is still in practice widely nowadays.
The scientists in the lab use air pressure to drive a solvent through the silica gel column, following up with column compression. After the sample is applied, the solvent will pass the sample through the column. And then we can collect the purified components or fractions. The process often takes about 5-10 minutes.
没有评论:
发表评论