Installation of the HPLC Column

The installation of the HPLC column should be carried out in the following three steps:

a. Unpack the HPLC column and confirm the type, size, date of manufacture, and solvent stored in the column.

b. Unscrew the sealing plug from the joints at both ends of the column and put it back in the box for spare.

c. According to the flow direction indicated on the HPLC column, connect the inlet end of the column to the outlet of the injection valve through the connecting pipe (if the conditions permit, it is recommended to use the guard column in front of the column); the outlet of the column is connected with the detector. The connecting pipe is a stainless steel pipe having an outer diameter of 1.57 mm and an inner diameter of 0.1 to 0.3 mm.

Both ends of the connecting tube have a hollow screw and a sealing ring for sealing. Always try to reduce the dead volume outside the column when taking over. After the connecting tube passes through the hollow screw and the pressure ring, insert it as far as possible into the end, then tighten the hollow screw clockwise until it stops, then use the wrench to continue to screw 1/4-1/2 turn clockwise, remember not to use too much force.

If the HPLC column is leaking after being pressurized by the mobile phase, use a wrench to continue to turn 1/4 turn clockwise until it does not leak.