Flash column is a sample pretreatment technology developed by the combination of liquid-solid extraction and column liquid chromatography. It is mainly used for sample separation, purification, and concentration which has been widely used in medicine, food, environment, commodity inspection, chemical, and other fields.
Principle
Flash column adopts the separation principle of liquid chromatography separation for selective adsorption and selective elution. The frequent used method is to pass the liquid sample solution through the adsorbent, retain the substance to be tested, and use the appropriate strength solvent to wash away the impurities, and then quickly elute the test substance with a small amount of solvent, thereby achieving the purpose of rapid separation, purification, and concentration.
Flash column adopts the separation principle of liquid chromatography separation for selective adsorption and selective elution. The frequent used method is to pass the liquid sample solution through the adsorbent, retain the substance to be tested, and use the appropriate strength solvent to wash away the impurities, and then quickly elute the test substance with a small amount of solvent, thereby achieving the purpose of rapid separation, purification, and concentration.
It is also possible to selectively adsorb the interfering impurities and let the measured substance flow out; or simultaneously adsorb the impurities and the tested substance, and then selectively elute the test substance with a suitable solvent.
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