Paper chromatography uses filter paper as an inert support. The filter paper fiber has a strong affinity with water, and can absorb about 22% of water, and 6 to 7% of the water is hydrogen bonded to the hydroxyl group of cellulose, which is difficult to remove under normal conditions. The affinity with organic solvents is very weak, so the general paper chromatography actually uses the binding phase of filter paper fibers as the stationary phase and the organic solvent as the mobile phase.
When the mobile phase passes the sample along the paper, the solute at the sample point is in water and the organic phase is continuously distributed. Part of the sample moves with the mobile phase and enters the solute-free zone. At this time, it is redistributed, and part of the solute enters the stationary phase (water phase) from the mobile phase. With the continuous movement of the mobile phase, various parts are continuously distributed according to their respective distribution coefficients, and move along the mobile phase, so that the material is separated and purified.
#Application And Operation Process Of Wet-strengthened Filter Paper#
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