Column chromatography is the most primitive chromatographic method, the stationary phase installed in a metal or glass column or fixed phase adsorption on the carrier made of the chromatographic column, the sample from the column head to the end of the column along the direction of separation.
Principle
High-performance liquid chromatography – HPLC, is based on the classical chromatography cited the theory of gas chromatography. HPLC technically changes the mobile phase to the liquid transported under high pressure. Chromatographic columns are filled with a special method of small particle size filler so that the column efficiency is much higher than that of classical liquid chromatography.
Application
HPLC is the most widely used chromatographic method and widely used in almost every field of quantitative and qualitative analysis. The high-performance liquid chromatography system is composed of mobile phase storage liquid bottle, infusion pump, sampler, chromatographic column, detector, and recorder. Its overall composition is similar to that of gas chromatography, but many adjustments have been made for its mobile phase as a liquid.
Feature
The infusion pump of HPLC requires constant and stable infusion volume, and the injection system requires convenient and strict switching. As the viscosity of the liquid mobile phase is much higher than that of gas, in order to reduce the column pressure, HPLC columns are generally thicker and much shorter than gas chromatographic columns.
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