How to Use Pipettes

Before Use

It is important to check the marks, accuracy class and graduation tails before using pipettes.

Imbibition

Use thumb and middle finger of your right hand to pinch the upper end of a pipette, then insert the lower end into the solution to a depth of 10 mm to 20 mm. Use your left hand to hold a rubber suction bulb and connect it to the upper end of the pipette. Suck the solution slowly (Notice: In case of not diluting the solution, the sucked solution cannot be reflowed to the original bottle) and use the index finger of your right hand to take the pipette out.

Turn the pipette horizontally in order to make the solution touch above the graduation and replace the inwall water. Discard the solution from the lower end of the pipette and repeat washing for three times. Finally, you can imbibe the solution to a level of 5 mm above the graduation and use the index finger of your right hand to press the upper end of the pipette immediately.

Adjust Liquid Level

Lift the pipette out of the liquid level and use filter paper to clean the outer wall water. Lose your index finger a little bit to let the solution flow out slowly, then press the upper end of the pipette. Take out of the pipette and insert it into the container to absorb the solution.

Original Data Record

Normally, the relevant original data record of the pipette can be given to two decimal places.

How to Use Pipettes