HPLC Column Silicone Matrix Properties and Scope of Application

Hawach introduced the properties and application range of HPLC column stationary phase in the silica gel matrix.

Silica gel matrix:

Silica gel, weakly acidic, has a strong memory, used to separate non-polar, weakly acidic compounds, oils, etc.;

C18, a silica-based uncapped reversed-phase C18 column with high bond and density, low loss, high recovery, etc.

C8 octyl, the retention of non-polar compounds is weaker than C18;

CN Cyano, a silica-based cyanopropyl column with moderate polarity for reversed phase or normal phase separation, suitable for carbohydrates and cationic compounds;

NH2 amino group, silica-based aminopropyl stationary phase, used for the separation of phenol and phenolic pigments, petroleum hexaphenol, sugars, drugs and metabolites;

PSA-propyl ethylenediamine, a stationary phase similar to NH2, PSA has two amino groups, which is more ion exchangeable than NH2, and PSA can also chelate with metal ions for extracting metal ions;

SAX strong anion exchange, bonded with quaternary ammonium salt functional groups, mainly used for the separation of weak anionic compounds;

COOH carboxyl group, the bonding functional group is a carboxylic acid, used for the separation of quaternary ammonium salts or other strong cations;

PRS propane sulfonic acid, the bonding functional group is a sulfonic acid, used for weak cations, widely used in the analysis of malachite green;

SCX strong cation exchange, bond, and the functional group is benzenesulfonic acid, used for separation of organic base compounds and biological macromolecule desalting.

HPLC Column Silicone Matrix Properties and Scope of Application