The precision instruments are the foundation of research and quality control labs all over the world. Pipettors are an essential tool for life science research. The piston achieves liquid suction and discharges through the telescopic movement of the spring.
Under the push of the piston, part of the air is discharged, and the liquid is sucked using atmospheric pressure, and then the air is discharged by the piston. When using a pipettor, operate with the elasticity of the spring to control the pipetting speed and force. Here, HAWACH discusses 15 tips to lengthen the pipettor service life.
1. When sucking liquid, be sure to release thumb slowly and steadily. Never let go suddenly, to prevent the solution from being drawn too fast and rushing into the pipettor to corrode the plunger and cause air leakage.
2. In order to obtain higher accuracy, the tip needs to draw the sample solution in advance, and then formally pipettor, because when the serum protein solution or organic solvent is drawn, a layer of “liquid membrane” will remain on the inner wall of the tip, causing too small discharge volume and produces errors.
3. Liquids with large concentration and viscosity will produce errors. To eliminate the error, the compensation amount can be determined by experiment. The compensation amount can be set by changing the reading with the adjusting knob.
4. Use the analytical balance to weigh the weight of the pure water and calculate it to correct the liquid picker. 1ml of distilled water weighs 0.9982g at 20℃.
5. It is undesirable to impact repeatedly the pipettor to tighten it. The long-term operation will loosen the internal parts and damage the pipettor.
6. Do not pipettor when the pipettor is not equipped with a pipettor tip.
7. When setting the range, please note that the set range is within the range of the pipettor, do not turn the button out of the range, otherwise, it will jam the mechanical device and damage the pipettor. The number is clearly in the display window and rotate to the desired range.
8. Pipettors are strictly forbidden to absorb highly volatile and highly corrosive liquids (such as concentrated acid, concentrated alkali, organic matter, etc.).
9. Do not use a large range of pipettors to remove small volumes of liquid, so as not to affect the accuracy. At the same time, if you need to remove a large amount of liquid outside the range, please use a transfer pipettor.
10. If not in use, adjust the range of the pipettor to the maximum scale to keep the spring in a relaxed state to protect the spring.
11. It is best to clean the pipettor regularly, use soapy water or 60% isopropyl alcohol, then use distilled water to clean, and dry naturally.
12. Before high-temperature disinfection, make sure that the pipettor can adapt to high temperatures.
13. Calibration can be performed in the environment of 20℃~ 25℃ by repeating the weighing of distilled water several times.
14. Check if there is any liquid leakage during use. After absorbing the liquid, hang it vertically and let it stand for a few seconds to see if the liquid level drops. If the liquid leaks, the reasons are: (1) whether the tip matches; (2) whether the spring piston is normal; (3) if it is a volatile liquid (as is the case with many organic solvents), it may be a problem of saturated vapor pressure. Aspirate the liquid several times before pipetting.
15. After the use of pipettor, hang it vertically on the pipettor holder, but be careful not to drop it. When there is liquid in the pipettor tip, do not place the pipettor horizontally or upside down to prevent the backflow of liquid from corroding the piston spring.
没有评论:
发表评论