When flash column works, the upper interface generally has a gas/liquid (with a certain pressure) to speed up the sample separation process. It operates at pressures of 50 -200 psi, as compared to HPLC, which operates at 1000s of psi, and UHPLC which operates above 10,000 psi. Flash column is the primary purification technique used in organic synthesis and industrial applications because it is fast (Flash!), it is easy to use and it is versatile.
The method of the flash column chromatography is to separate the components from the mixture and purify it. In short, fast column chromatography makes samples go through gel-filled columns, and the gels help make the separation. The founders of the fast column chromatography at first wanted to find a method to overcome the shortcomings of long column chromatography, such as time-consuming and low recovery.
The gel used initially for packing columns is silica gel, which is still widely used. People push solvents through silica gel flash columnsby air pressure. And then they add the samples in it and push samples through the columns. The components needed to be collected and purified will appeared during the process of the elution.
Since the silica gel, flash column chromatography is widely used in organic chemistry, the guidelines are important when you try to get better sample quantity results. The flow rate depends on different column length and width, and the properties of the gel as well.
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