Hawach Scientific provides HPLC Columns with a variety of particle sizes and specifications to meet all separation requirements, including higher resolution, enhanced sensitivity, faster analysis, and consistent performance. All these are guaranteed by strictly selected and qualified materials such as spherical and pure silica gel, a highly cross-linked rigid spherical polystyrene/divinylbenzene (PS/DVB) non-porous scraping packing surface, a hydrophilic polymer layer, which can effectively eliminate the nonspecific absorption of antibody protein and improves the column efficiency and recovery.
Improvements of HPLC Columns
Hawach Scientific gains some highlights in capping, ultra-pure, silica gel chromatographic columns, which can significantly reduce peak trailing by means of universal gradient and C18 selectivity. With excellent resolution, efficiency and sensitivity, Hawach HPLC column can give the customer confidence in the accuracy and quality of the analysis data.
Hawach Scientific gains some highlights in capping, ultra-pure, silica gel chromatographic columns, which can significantly reduce peak trailing by means of universal gradient and C18 selectivity. With excellent resolution, efficiency and sensitivity, Hawach HPLC column can give the customer confidence in the accuracy and quality of the analysis data.
The highly homogeneous, dense-packed weak cationic exchange layer is chemically bonded to the hydrophilic polymer coating surface. And the complete characterization of monoclonal antibodies contains the identification and monitoring of acidic and alkaline subtypes, which is beneficial to provide a higher degree of separation and excellent column property in peak shape.
Experimental Matters Needing Attention
HPLC columns must undergo correct aging before the analysis and operation, and Hawach has tested and aging each column. The eluents recommended for use on such columns are low conductivity buffers, such as citric acid or tartaric acid buffers ( or their mixed solution), pH ranges from 2.5 to 6.5, in which ethylenediamine is used as eluting cations.
HPLC columns must undergo correct aging before the analysis and operation, and Hawach has tested and aging each column. The eluents recommended for use on such columns are low conductivity buffers, such as citric acid or tartaric acid buffers ( or their mixed solution), pH ranges from 2.5 to 6.5, in which ethylenediamine is used as eluting cations.
The salicylic acid buffer must not be used because salicylic acid decomposition products change the properties of the stationary phase. The eluent should be deaerated to avoid detection and pumping problems. Besides, be sure to check for microbial growth before you start using the system, or your column will clog and the column pressure will rise to an unacceptable level.
没有评论:
发表评论