Make sure that the pipette, the head, and the liquid are at the same temperature before liquid transferring at first. When sucking the liquid, you should hold the upper part of the pipette with four fingers together and press the button on the top of the plunger rod with your thumb. And then keep the pipette vertical, insert the head 2-3 mm below the liquid level. Release the button slowly, and stay for one or two seconds to suck liquid. Slide the head of the pipette out of the container along the wall. Finally, press the push rod of the head into the waste tank.
There are two methods of liquid transfer, as described below.
One is forward pipetting. Press the button with your thumb to the first stop spot, and then release the button back to the original spot slowly. Next, press the button to the first stop point to discharge the liquid, and then press the button to the second stop point to blow out the residual liquid. At last, release the button.
The other is reverse pipetting. Generally, the method is used to transfer liquid with high viscosity, biologically active, easily foaming. The principle is to suck liquid beyond the set range, and not blow out the residual liquid when transferring. First, press the button to the second stop point, and release the button to the original state slowly. Then press the button to the first stop point to discharge the liquid with the set range, and keep the button pressed and held at the first stop point. Remove the head with residual liquid and discard it.
Selection Knowledge of Pipette Tips
Significance of Selection
When choosing and purchasing pipettes, the users usually have taken a lot of factors such as precision, handle, brand, suction head adaptability and so on into consideration. In fact, the pipette tips will have a lot of impact on the accuracy of liquid transfer, because the tip bears the philosophy of perfect function, steady performance, and delicate appearance. Innovative handling and packaging of pipette tips are beneficial to overcome common problems of automatic pipette tips, such as static stacking and retention of residues.
When choosing and purchasing pipettes, the users usually have taken a lot of factors such as precision, handle, brand, suction head adaptability and so on into consideration. In fact, the pipette tips will have a lot of impact on the accuracy of liquid transfer, because the tip bears the philosophy of perfect function, steady performance, and delicate appearance. Innovative handling and packaging of pipette tips are beneficial to overcome common problems of automatic pipette tips, such as static stacking and retention of residues.
没有评论:
发表评论