2019年11月17日星期日

Pipette Operation And Common Mistakes

Pipette is a perfect tool to transport a measured volume of liquid. It is widely used as a media dispenser in labs. For different purposes and needs of accuracy and precision, we can find pipettes in several designs in the market, glass and polyethylene ones. Adjustable and electronic pipettes are available too.
The pipette is always needed wherever the precise transfer of liquid is required in the lab. Regardless of what type the pipette you have, you should handle them with care. Placing the pipette 1/4 of an inch from the bottom of the container is the right way to prevent damage when you draw the liquid. You’d better tap the side of the pipette to remove excess droplets very gently when the required volume of the liquid is drawn up. Always hold the pipette at a 10-20 degree angle when you dispense. Never blow through a pipette and try to remove extra liquid.
To make sure the pipettes working accurately and also to prevent pollution from previous contents, you should clean the them after use.
Operation Procedures of Pipette
To do a correct liquid operation using a normal pipette, there are some operating steps for follow, first, connect the suction head, then press the piston exhaust, insert the suction head approximately 2 mm below the liquid level, next, inhale liquid and transfer to the destination container, finally, push off the suction head. It takes about 10 seconds for the whole process.
Maintenance Procedures of Pipette
In experimental operation, when there is liquid in the suction nozzle of the pipette, do not place the it horizontally or inversely to prevent the liquid flowing into the piston chamber to corrode the pipette piston.
Besides, use the pipette correctly to absorb and drain liquid to achieve high precision; when it occurs leakage in operation, stop for one to three seconds after suction to see whether the liquid level in the suction head drops, if the liquid level drop, firstly to check whether there is a problem with the suction head, if it is, just replace the suction head, if not, call for professional maintenance personnel to repair.

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