In order to avoid the fruits of your labor being eaten away by pollutants, and to ensure that the culture medium and additives are not sources of pollution, it is necessary for us to come up with powerful weapons to sterilize. However, autoclaving commonly used in laboratories is not ideal for many liquids, because this process may destroy key components and growth factors required for normal cell growth. In this case, filtration has become the preferred method of sterilizing cell culture media and additives.
In the laboratory, culture contamination is one of the most disruptive things. Often, bacterial, fungal, or yeast contaminants unknowingly invade your culture, making your research efforts in the past few days or even weeks to a halt. Unfortunately, the medium used for cell culture is particularly suitable for the growth of pollutants. They are nutritious, sweet and delicious.
In order to avoid the fruits of your labor being eaten away by pollutants, and to ensure that the culture medium and additives are not sources of pollution, it is necessary for us to come up with powerful weapons to sterilize. However, autoclaving commonly used in laboratories is not ideal for many liquids, because this process may destroy key components and growth factors required for normal cell growth. In this case, filtration has become the preferred method of sterilizing cell culture media and additives.
For filtration and sterilization, a syringe filter is of course the first choice. This kind of disposable equipment is ready to use and does not require membrane replacement and cleaning. It can easily purify or sterilize various solutions, including cell culture media and microbial culture media, reagents and additives, protein solutions and buffers. Wait. However, there are many types of syringe filters on the market to choose from. How to reduce the filtration time while ensuring the sterilization effect requires consideration from the following aspects.
1.Quality. Since it is sterilization, we certainly hope that the syringe filter can remove as much bacteria as possible without introducing other substances, such as bacterial endotoxins or chemical precipitates. The syringe filter are sterilized by gamma irradiation to eliminate cytotoxic residues associated with ethylene oxide sterilization. At the same time, the product has been tested and optimized, free of endotoxins, and low precipitation, ensuring that unnecessary substances will not be introduced into the filtered liquid.
2.Filter membrane. There are many kinds of filter membranes used in syringe filters. We must consider select the both chemical compatibility and filtration time. For aqueous solutions such as cell culture media, hydrophilic membranes are the best choice. This membrane has a low protein binding rate, minimizes sample loss, and has a fast flow rate and high throughput, which is particularly suitable for the filtration of culture media.
3.Specifications. After determining the filter and membrane, the selection of specifications is relatively easy. Take Pall as an example. When processing samples below 125 ml, you can choose a 25mm syringe filter. If the sample volume is larger, you can choose a syringe filter with a diameter of 32mm or 37mm.
These filters also have different pore sizes to choose from. To remove bacteria, a 0.2 μm filter is the most suitable choice. If you want to remove even mycoplasma, you can choose a 0.1 μm filter. For pre-filtration or particle removal, you can choose a 0.45 μm filter membrane.
In view of this, in the laboratory to filter and sterilize, you can choose a syringe filter. This syringe filter is suitable for filtration of various aqueous solutions. Of course, if it is the filtration of organic phase solution, then you need to choose other membranes.
The housing material of the needle filter is made of high-quality sanitary polypropylene material, and the product structure is precisely designed to ensure smooth filtration, rational internal space, and very low residual rate, thereby reducing the waste of samples. In HPLC analysis, the chromatographic column packing has a small particle size and is easy to be blocked by impurity particles. Therefore, the sample and solvent need to be pre-filtered to remove particulate contaminants and protect the instrument.
The ion chromatography commonly used in the environmental analysis also requires that no inorganic contaminants be introduced in sample pretreatment. Syringe filters is ideal for HPLC analysis and IC analysis. Filtering sample solutions is an important step in the sample pretreatment process. One of the disadvantages of traditional filters is that they are easy to blast. This product is specially designed.
The edge of the filter is threaded, which plays a non-slip effect. The user-friendly design makes the operator handy, stable filter membrane quality, zero difference between batches, ensuring the consistency of analysis results, and clear specifications The logo eliminates the trouble of confusion. The needle filter has a beautiful appearance, a simple and reasonable structure, and a polished surface and inside.
It has the characteristics of low cost, small size, large filtering area, fast filtering speed, low blocking rate, acid and alkali corrosion resistance, and long service life. Participating in different precision filter elements (such as PP filter element, wire wound element, folding filter element, ceramic filter element, etc.) can remove various fine particles, colloids, metals, bacteria, residual chlorine and other impurities in water or air.
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