Common Types Of HPLC Columns

Depending on the packing of the HPLC column. The following column types are available on the market:

1. Silica gel HPLC column: Silica gel is the earliest chromatographic column packing used in liquid chromatography. It has a wide range of applications. However, it can only be used under the condition of pH 2.0-7.5, and the use temperature must be less than 60 degrees.

2. Chemically bonded stationary phase HPLC column

2.1 Non-polar bonded HPLC columns: the most widely used HPLC columns C1 to C22. Among them, C18 is the most commonly used non-polar reversed-phase column, and it has a wide range of applications. And the separation effect is good.

2.2 Polar-bonded HPLC column: commonly used amino (NH2) HPLC column, cyano (CN) HPLC column, diol group (DIOL), ion HPLC column

2.2.1 The selectivity of the amino column first appears as a strong hydrogen bonding ability, then in the acidic medium, the weak anion exchange, the tertiary amine and the hydroxyl of the grain interact, using acetonitrile: water system It is possible to separate a flash monosaccharide, a disaccharide, a polysaccharide, and the like.

2.2.2 The separation characteristics of the cyano column are similar to those of the silica gel HPLC column, but the polarity is relatively weak. The advantage is that the gradient elution or the composition of the mobile phase changes rapidly.

2.2.3 The diol-based bonding HPLC column is generally a hydrolysis product of a glycerol-oxypropylsilane-bonded phase, which has a good separation effect on organic acids and certain high polymers and can also be used for the water volume of certain proteins. Chromatography.

3. Polymer ball column.

4. A column coated with a polymer.

5. A column of a particulate porous carbon filler.

Common Types Of HPLC Columns