How to Clean the HPLC Columns

The reversed-phase HPLC columns may be repeatedly injected into a sample containing a strongly retained material, and in particular, the sorbent components such as large molecular weight or hydrophobic biological flow proteins and strong alkaline substances may be adsorbed on the silica medium.

In addition, some mobile phase additives such as ion-pairing test reagents and reactants can adsorb on the filling surface and change their properties. Contaminated columns can cause peak shape differences, poor retention of repeatability, high back pressure, and baseline drift. Typically, these columns can be cleaned using organic solvents or agents capable of destroying contaminants and binder phases or silica surfaces.

The users should be careful when using reagents that are corrosive or destructive to the binding phase. Additionally, the use of sample preparation minimizes contact between the HPLC columns and undesired materials to reduce contamination. We suggest using the HPLC protect column as pre-filter. Use Pre-filter, HPLC protect column, can reduce pollution from contamination and possibly store the analytical HPLC columns.

How to Clean the HPLC Columns