Operation Instruction of Three Different SPE Cartridges

The solid-phase extraction (SPE) devices are extremely popular equipment widely used in the sample pre-treatment technology. Among them, the SPE cartridge plays a significant role in the sample pretreatment process. Here are three different kinds of SPE cartridges below that could meet the various demands of the customers.

Instruction of Reversed-phase SPE Cartridge

The reversed-phase (RP) SPE cartridge is used to extract non-polar samples from polar matrices.

The first step is activation – rinse the cartridge with 3-5 ml of methanol or deionized water, which could keep the cartridge moist. Next, the sample needs to be evenly passed through the cartridge at a suitable flow rate. The third step is cleaning, which indicates the cartridge with the adsorbed sample has to be eluted with 5 ml of a polar solvent. (commonly used pure water) the last step is to elute the sample in batches with 1-5 ml of non-polar solvent to the collection container.

Instruction of Normal-phase SPE Cartridge

The normal-phase (NP) SPE cartridge is suitable for extracting polar samples from non-polar matrices. The first step is to rinse the cartridge with 3-5 ml of non-polar solvent. The next steps are just like using the RP SPE cartridge but only to clean the cartridge with a non-polar solvent and to elute the sample with a polar solvent.

Instruction of Ion-exchange SAX SPE Cartridges SPE Cartridge

The ion exchange SPE cartridge is available for extracting charged samples. The cartridge is rinsed with 3-5 ml of deionized water or low ionic strength buffer. It uses a 5 ml deionized water / or low ionic strength buffer to clean the cartridge itself and elute the sample with 1-5 ml of high ionic strength buffer.
Operation Instruction of Three Different SPE Cartridges