Reliable flash columns play a decisive role in a good chromatographic separation method, which ensures complete separation of valuable compounds and specific impurities. Choosing the right flash column is the most critical part of the purification process. There are two aspects should be carefully considered to select an appropriate flash column.
Stationary Phase
To determine the stationary phase of the flash column, there are three aspects should be considered, including matrix type, bonded phase, and matrix parameters. The commonly used matrix materials are divided into three categories – inorganic materials, organic materials, and composite materials. Silica, with high mechanical strength and thermal stability, is the most widely used inorganic matrix in the flash column. It holds an important position in the separation and purification of compounds in the normal phase purification system. Nevertheless, its stability is relatively poor under alkaline conditions.
The organic matrix is widely applied in the separation and purification of biomolecules such as proteins and sugars. As for the bonded phase, different separation types of stationary phases could be formed by bonding different groups to the matrix, such as stationary phase, reverse phase, ion exchange, SEC, and the like. The matrix parameters, including the shape, particle size, and pore size of the matrix, are selected in terms of the specific needs of the purification.
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