The working principle of the pipette is that the piston moves through the telescopic movement of the spring to achieve aspiration and discharge. Under the push of the piston, part of the air is exhausted, and the liquid is sucked in at atmospheric pressure, and then the air is pushed out by the piston to discharge the liquid. When using a pipette, it can be operated in accordance with the flexibility of the spring, which can control the speed and strength of pipetting.
Operation steps
A pipette is an indispensable tool for the experimental operation. The operators should use it correctly, or it will cause the error and even experiment failure.
A pipette is an indispensable tool for the experimental operation. The operators should use it correctly, or it will cause the error and even experiment failure.
1. Tip assembly
The pipette sleeve is pressed down firmly and can be rotated to a small extent when needed.
Don’ts: Hit the tip with force, which will cause damage to the tip or even wear of the pipette sleeve, affecting its sealing effect.
2. Volume setting
Please select the correct pipette before the operation. When adjusting from small to large volume, it will rotate continuously in the direction of the required volume, and reach back to the required volume after reaching more than 1/3 of the required volume. By doing so is to guarantee the best accuracy. When adjusted from large to small, which is the normal adjustment method, it is directly rotated continuously to the required volume.
3. Rinse
Repeat the aspirate and drain two to three times with the same sample, and provide the same contact surface for each aspiration to ensure the consistency of operation.
Don’ts: Please do not rinse the high temperature or low-temperature liquid.
4. Tip immersion angle
When aspirating, keep it vertical and do not make the inclination angle exceed 20°.
5. Tip immersion depth
0.1-10μL: 1-2mm; 10-200μL: 2-3mm; 200-2000μL: 3-6mm; over 2000μL: 6-10mm
The pipette sleeve is pressed down firmly and can be rotated to a small extent when needed.
Don’ts: Hit the tip with force, which will cause damage to the tip or even wear of the pipette sleeve, affecting its sealing effect.
2. Volume setting
Please select the correct pipette before the operation. When adjusting from small to large volume, it will rotate continuously in the direction of the required volume, and reach back to the required volume after reaching more than 1/3 of the required volume. By doing so is to guarantee the best accuracy. When adjusted from large to small, which is the normal adjustment method, it is directly rotated continuously to the required volume.
3. Rinse
Repeat the aspirate and drain two to three times with the same sample, and provide the same contact surface for each aspiration to ensure the consistency of operation.
Don’ts: Please do not rinse the high temperature or low-temperature liquid.
4. Tip immersion angle
When aspirating, keep it vertical and do not make the inclination angle exceed 20°.
5. Tip immersion depth
0.1-10μL: 1-2mm; 10-200μL: 2-3mm; 200-2000μL: 3-6mm; over 2000μL: 6-10mm
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