Short for High-performance liquid chromatography, HPLC is an analytical chemistry technique used to separate, quantify and identify the components which are in a mixture. In the HPLC technique, the pumps push some liquid solvents which contain a mixture of the through a HPLC column which contains solid absorbent materials.
The principle of HPLC is easy to understand. Each of the components in the sample will interact with the absorbent material in a different way, which will cause different flow rates in the various components, and the components will be separated as they flow out of the HPLC column.
The HPLC column is called the stationary phase, which is made from different substances. You can find that silica is popular in the absorbent material, as it’s highly compact in nature. The full lines of Hawach HPLC column products will help you to get faster separation for similar samples. The wonderful performance of the HPLC columns will get your HPLC method greatly improved.
Common terms for HPLC
1. Chromatogram: a graph of the response signal generated by the HPLC column effluent when passing through the detector system versus time or mobile phase effluent volume, or a paper chromatogram (ch or paper layer spot observed by appropriate methods), The distribution map of the band.
2. Chromatographic peak: The differential curve of the response signal generated when the effluent from the HPLC column passes through the detector system.
3. Peak base: The straight line connecting the start and end of the peak (CD in Figure 1).
4. Peak height (h): The distance from the maximum point of the chromatographic peak to the peak bottom (BE in Figure 1).
5. Peak width (W): The distance between the two points where the tangent line and the bottom of the peak intersect at the inflection points on both sides of the peak (F, G in Figure 1) (KL in Figure 1).
6. Half-peak width (W h / 2, the peak with a half-height): Make a straight line parallel to the bottom of the peak through the midpoint of the peak height. The distance between this straight line and the two points that intersect at both sides of the peak (Figure 1 HJ).
7. Peak area (A): the area between the peak and the peak bottom (CHEJDC in Figure 1).
2. Chromatographic peak: The differential curve of the response signal generated when the effluent from the HPLC column passes through the detector system.
3. Peak base: The straight line connecting the start and end of the peak (CD in Figure 1).
4. Peak height (h): The distance from the maximum point of the chromatographic peak to the peak bottom (BE in Figure 1).
5. Peak width (W): The distance between the two points where the tangent line and the bottom of the peak intersect at the inflection points on both sides of the peak (F, G in Figure 1) (KL in Figure 1).
6. Half-peak width (W h / 2, the peak with a half-height): Make a straight line parallel to the bottom of the peak through the midpoint of the peak height. The distance between this straight line and the two points that intersect at both sides of the peak (Figure 1 HJ).
7. Peak area (A): the area between the peak and the peak bottom (CHEJDC in Figure 1).
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