Advantages
1) Compared with atmospheric pressure chromatography, the separation efficiency is higher;
2) Natural traditional advantages in protein purification;
3) It is more economical than high-pressure preparative chromatography, the instrument requires less configuration, and the user can install the column by himself;
4) The use of pre-packed columns can increase the rate and save time and manpower;
5) It has a direct relationship with TLC. Using TLC can quickly establish a separation method;
6) More solvent saving than traditional column method;
7) Samples that are not easily separated by conventional manual column passing can be achieved by the column passing machine.
1) Compared with atmospheric pressure chromatography, the separation efficiency is higher;
2) Natural traditional advantages in protein purification;
3) It is more economical than high-pressure preparative chromatography, the instrument requires less configuration, and the user can install the column by himself;
4) The use of pre-packed columns can increase the rate and save time and manpower;
5) It has a direct relationship with TLC. Using TLC can quickly establish a separation method;
6) More solvent saving than traditional column method;
7) Samples that are not easily separated by conventional manual column passing can be achieved by the column passing machine.
So how do you choose a FLASH column?
1. Convenience
Compared with the traditional column method, the FLASH column is fast and easy to operate. Convenience is mainly reflected in two aspects, one is the user-friendliness and the other is the efficiency of separation and purification.
1. Convenience
Compared with the traditional column method, the FLASH column is fast and easy to operate. Convenience is mainly reflected in two aspects, one is the user-friendliness and the other is the efficiency of separation and purification.
1) User-friendliness: First, check the hardware facilities of the FLASH column; second, the software operation experience; whether the entire process design is reasonable, including whether the software operation is smooth and whether it is convenient to check the experimental progress, record the experiment and so on.
2) In terms of separation and purification efficiency, in general, the efficiency of the FLASH column is definitely much higher than that of the manual column.
In terms of separation time, it is generally passed by machine, and a sample is good for half an hour, but it may take several hours or even 1 day by manual; In addition, in terms of the separation effect, some samples, such as two points on a TLC board, are close to each other and cannot be separated manually, and can be separated by a machine, which also virtually improves the experimental efficiency.
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