As a unique chromatography technique that pushes solvent through the column uses by compressed gas or a pump, flash column chromatography is highly appreciated as an advance technique because it makes flow rates of the solvent much faster than the gravity flow.
Developed in the 1970’s, flash column chromatography was upgraded from long column chromatography technology in the lab. At that time, the scientists were tired of long column chromatography, because it’s kind of time-wasting with poor results. But flash column chromatography can not only provide better separation but also reduce the running time of the sample separations. The whole process usually takes about 5-10 minutes.
The silica gel is not only the gel which was first used in the column, but it is widely employed still nowadays. The air was pressured to drive the solvent through the silica gel column and compress it. After the sample is applied, and the same solvent is used to pass the sample through the column. The solvent can be different sometimes. And then, it’s the time we can collect the purified component, small fractions emerge first, and the larger ones go after.
Flash is a fast separation mode for preparative chromatography, which uses optimized pre-packed low and medium pressure columns for chromatographic separations. Flash is considered to be low- and medium-pressure separation chromatography, and its resolution is correspondingly lower than HPLC (high-performance liquid chromatography, or high-pressure liquid chromatography).
Flash technology was developed in 1978 and is used to purify organic compounds. Compared to traditional column chromatography, it is a fast and inexpensive technology. Flash separation and purification technology are now widely used in various applications such as drug development, sample purification, and natural product purification. Because of its low cost and simplicity, FLASH is one of the chromatography separation and purification tools that cannot be replaced by preparative HPLC.
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