2019年12月15日星期日

Proper Use Of Pipette

1. Adjusting range
When adjusting the measuring range, if you want to adjust from large volume to small volume, you can rotate the knob anticlockwise according to the normal adjustment method; but if you want to adjust from small volume to large volume, you can first rotate the scale knob clockwise to the scale beyond the measuring range, and then callback to the set volume, so as to ensure the highest accuracy of measurement. In this process, do not turn the button out of the range, otherwise, the internal mechanism will be stuck and the pipette will be damaged.
2. Assemble the pipette head (suction nozzle)
When the pipette head is put on the pipette, the correct method is to insert the pipette (device) vertically into the pipette head and turn it slightly to the left and right with a little force to make it tightly combined. If it is a multi-channel (such as 8 or 12 channels) pipette, the first channel of the pipette can be aimed at the first pipette head, then it can be inserted obliquely, and it can be clamped by shaking forward and backward. The mark of the pipette head clamping is slightly more than the O-ring, and the connection part can be seen to form a clear sealing ring.
3. Method of liquid transfer
Before liquid transfer, make sure that the pipette, pipette head, and liquid are at the same temperature. When sucking the liquid, keep the pipette vertical and insert the pipette head 2-3mm below the liquid level. Before sucking the liquid, you can first suck and put liquid several times to wet the sucking nozzle (especially when you want to suck liquid with viscosity or density different from water). At this time, two methods of liquid transfer can be adopted.

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