The liquid chromatography is a kind of high-precision, high-sensitivity qualitative and quantitative analysis instrument. Due to the quite precise components of LC and high accuracy and sensitivity of detection, the purity requirements of the sample and mobile phase are also quite high in the process of detecting samples.
Three types of filtration membrane in LC
Before the LC detection, the mobile phase must be filtered to remove impurities after the mobile phase is selected. The solvent filter is mainly used and equipped vacuum pump to speed up the filtration rate. Among them, the filter membrane plays a key role. Depending on the mobile phase solution, the type of membrane used in the solvent filter varies. The filter membrane is mainly divided into two types: water-based and organic-type membranes. The pore size of these two types of filter membranes is very small and the filtering effect is good.
Before the LC detection, the mobile phase must be filtered to remove impurities after the mobile phase is selected. The solvent filter is mainly used and equipped vacuum pump to speed up the filtration rate. Among them, the filter membrane plays a key role. Depending on the mobile phase solution, the type of membrane used in the solvent filter varies. The filter membrane is mainly divided into two types: water-based and organic-type membranes. The pore size of these two types of filter membranes is very small and the filtering effect is good.
Water-based membranes are suitable for water-based solutions, are not resistant to organic solvents, have high chemical and thermal stability, fast flow rates, strong resistance to acids and alkalis, high mechanical strength, and low protein adsorption.
Organic membranes are hydrophobic membranes, suitable for organic solvents and air filtration, which is acid and alkali resistant, antioxidant, with high-temperature resistance, good strength, and stable chemical properties. It is not only suitable for aqueous solutions containing acid and alkali, but also for organic solvents such as alcohols, hydrocarbons, lipids, phenols, ketones, and other organic solvents.
In addition, there is a filter membrane made of aliphatic nylon, which has good hydrophilicity and resistance to appropriate concentrations of acids and bases. It is not only suitable for aqueous solutions containing acid and alkali, but also for organic solvents, such as alcohols and hydrocarbons. , Ethers, Esters, ketones, benzene and benzene homologs, dimethylformamide, dimethylsulfoxide, etc., which is one of the most widely used microporous filter membranes.
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