2020年3月10日星期二

Basic Knowledge Of HPLC Column

HLPC means High-Performance Liquid Chromatography, which is also known as “High-pressure Liquid Chromatography”, “High-speed Liquid Chromatography”, “High separation Liquid Chromatography”, “modern column Chromatography”, etc.
HPLC is an important branch of chromatography, using the high-pressure infusion system to put the single solvents with different polarity or different portion of mixed solvents and mobile phases into a stationary phase chromatographic column, in which each component is separated and then entered the detector for testing, so as to realize the analysis of the sample.
Column Specification:
This is normally shown as column length * inside diameter. For example: 150*4.6, 50 * 2.1, etc. Generally, 4.6 of diameter is used on the conventional detector and 2.1 is used with MS detector.
Types of HAWACH HPLC Column
There are 2 kinds of HAWACH HPLC columns:
Xcheoma series: with many types of fixed phase; with complete varieties and high quality. C18-Aqueous; C18-universal;C18-low pH; C18-Alkaline; C8-universal; C8-Low pH; C8-Fluorine; C4; C30; C1;Phenyl; PAH; etc
Echroma series: less column type, slightly cheaper. C18: 5um; C8: 5um.
Characteristics of HPLC Column
1. High column quality recognition; imported chromatographic filler with high purity and goof uniformity
2. High column efficiency and high peak capacity.
3. Excellent batch stability.
4. Multiple types of chromatographic columns, there are many different types of C18 column and series.
Complete column specifications, particularly, X series column has various fillers, different varieties of column length and internal specifications, which can meet the requirements of the majority of analysis experiments.

没有评论:

发表评论