Chromatography, also known as chromatography or chromatography, is a physicochemical analysis method that uses the difference between the forces (distribution, adsorption, ion exchange, etc.) between different solutes (samples) and the stationary phase and mobile phase. When the two phases move relatively, each solute is equilibrated multiple times between the two phases, so that the solute is separated from each other. The following related concepts are commonly found in the use of HPLC columns.
Chromatogram related concepts
Chromatogram: A plot of the response signal generated by the HPLC column effluent as it passes through the detector system versus time or carrier gas volume.
Chromatographic peak: A differential curve of the response signal produced by the column as it exits the detector system.
Baseline: The line connecting the start and end of the peak.
Peak height: The distance from the peak maximum to the peak baseline.
Peak Width: The distance between the tangent at the inflection point on both sides of the peak and the point at which the peak baseline intersects.
Half-width: The line passing through the midpoint of the peak height equal to the bottom of the peak, the distance between the line and the two sides of the peak.
Peak area: The area between the peak and the peak baseline.
Chromatographic peak: A differential curve of the response signal produced by the column as it exits the detector system.
Baseline: The line connecting the start and end of the peak.
Peak height: The distance from the peak maximum to the peak baseline.
Peak Width: The distance between the tangent at the inflection point on both sides of the peak and the point at which the peak baseline intersects.
Half-width: The line passing through the midpoint of the peak height equal to the bottom of the peak, the distance between the line and the two sides of the peak.
Peak area: The area between the peak and the peak baseline.
Related concepts of chromatographic processing
Data acquisition: During the process of collecting data, the signal output by the analytical instrument is converted from an analog signal to a digital signal in the collector. The digital signal is sent to the N2010 chromatography station and saved in the signal data file.
Integration: Integration is to determine the peak from the signal curve and calculate its size. Points are essential for quantitative calculations. When integrating the N2010 Chromatography Workstation, first identify the start and end times of each peak, and mark the points with the “|” symbol, find the vertices of these peaks, determine the retention time, establish a baseline, and calculate the peak area, peak height, and peak width. These processes are controlled by integral parameters and manual integration event tables.
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