Cleaning and storage of HPLC column
Have a good understanding of the sample made, and wash it with the mobile phase that has the strongest eluting capacity for the sample. For silica gel HPLC column, first wash away the polar impurities with methanol, then use dry dichloromethane and n-heptane 100 — 20OraL is activated in sequence. For the bonds and phase columns, it is generally washed with methanol-chloroform-methanol-water in order. Each column has a specific cleaning method, which must be read in accordance with its instructions.
Remove impurities and salts before storage, use a suitable storage solvent to avoid drying out of the column bed, avoid mechanical vibration, prevent bacterial growth, and pay attention to the storage temperature.
For HPLC column Hawach have different types. And different types have different request for cleaning and storage. We will provide you product instruction for you with our product. And if you have any question for that we are going to solve your problem as fast as we can. Choose Hawach is going to have a good service experience. And we are happy to cooperate with you.
The HPLC column system consists of a reservoir, a pump, an injector, a chromatographic column, a detector, and a recorder. The mobile phase in the reservoir is driven into the system by a high-pressure pump, and the sample solution enters the mobile phase through the sampler. Different distribution coefficients, when making relative movement in the two phases, after repeated repeated adsorption-desorption distribution processes, the components have a large difference in moving speed, and are separated into individual components and flow out from the column in turn. When passing the detector, the sample concentration is converted into an electrical signal and transmitted to the recorder, and the data is printed in the form of a map.
The temperature of the ordinary bonded stationary phase of HPLC column with silica gel as the carrier usually does not exceed 35 ° C. In order to improve the separation effect, the temperature of the column can be appropriately increased, but it cannot exceed 60 ° C. If you want to know more information about HPLC column don’t hesitate to contact us.
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