In the narrow sense, FLASH refers to the preparation of a liquid chromatography system using a low-pressure short column that generates pressure with compressed gas and accelerates the elution rate of the mobile phase. In the broad sense, FLASH refers to all low-pressure liquid chromatography systems for infusion.
Development of flash column
After more than 20 years of development, FLASH chromatography has been widely used as a conventional purification and separation equipment. FLASH chromatography was born out of open glass column chromatography and is mainly used for the separation and purification of natural products and organic synthetic products.
After more than 20 years of development, FLASH chromatography has been widely used as a conventional purification and separation equipment. FLASH chromatography was born out of open glass column chromatography and is mainly used for the separation and purification of natural products and organic synthetic products.
Classical FLASH chromatography consists of a glass column, a liquid storage bottle, and a compressed air regulating valve. This type is low cost and easy to observe, but has low pressure and is easy to break. After improvement, a classic FLASH chromatography system was formed. It consists of an infusion pressure tank, a flow regulating valve, a flash preloading column, and a sample loading device. The equipment is compact, high pressure, easy to use, and highly reproducible. Classic FLASH chromatography still uses air pressure, with a pressure range of about 0-7 bar, a column length of about 15 cm, and a particle size of 40-60. Load solids and liquids and collect fractions manually.
Recently, due to the intensification of scientific research competition and the increase of labor costs in laboratories, a higher degree of automation and separation speed is required, and automated FLASH chromatography has been generated. The instrument also has a gradient elution system, a column switching system, and an automatic sampling system. The conditions can be selected and optimized to achieve unmanned operation, which greatly reduces labor costs and speeds up development.
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